Identification, origin, and evolution of leaf nodulating symbionts of Sericanthe (Rubiaceae)

Show simple item record Lemaire, Benny Robbrecht, Elmar Van Wyk, Abraham Erasmus (Braam) Van Oevelen, Sandra Verstraete, Brecht Prinsen, Els Smets, Erik Dessein, Steven 2012-03-05T09:41:48Z 2012-03-05T09:41:48Z 2011-12
dc.description.abstract Bacterial leaf symbiosis is an intimate association between bacteria and plants in which endosymbionts are housed within leaf nodules. This phenomenon has been reported in three genera of Rubiaceae (Pavetta, Psychotria, and Sericanthe), but the bacterial partner has only been identified in Psychotria and Pavetta. Here we report the identification of symbiotic bacteria in two leaf nodulating Sericanthe species. Using 16S rRNA data and common housekeeping genetic markers (recA and gyrB) we studied the phylogenetic relationships of bacterial endosymbionts in Rubiaceae. Endosymbionts of leaf nodulating Rubiaceae were found to be closely related and were placed as a monophyletic group within the genus Burkholderia (β-Proteobacteria). The phylogenetic analyses revealed a pattern of strict host specificity and placed the two investigated endosymbionts at two distinct positions in the topology of the tree, suggesting at least two different evolutionary origins. The degree of sequence divergence between the Sericanthe endosymbionts and their relatives was large enough to propose the Sericanthe endosymbionts as new species (‘Candidatus Burkholderia andongensis’ and ‘Candidatus Burkholderia petitii’). In a second part of this study, the pylogenetic relationships among nodulating and non-nodulating Sericanthe species were investigated using sequence data from six chloroplast regions (rps16, trnG, trnL-trnF, petD, petA-psbJ, and atpI-atpH). Overall, genetic variation among the plastid markers was insufficient to enable phylogenetic estimation. However, our results could not rule out the possibility that bacterial leaf symbiosis originated once in a common ancestor of the Sericanthe species. en
dc.description.librarian nf2012 en
dc.description.sponsorship The King Léopold III Fund and the Fund for Scientific Research – Flanders (FWO), the Research Program of the Fund for Scientific Research – Flanders (Belgium) (FWO – Vlaanderen, G.0343.09N) and the K.U. Leuven (OT/05/35). en_US
dc.description.uri en_US
dc.identifier.citation Lemaire, B, Robbrecht, E, Van Wyk, B, Van Oevelen, S, Verstraete, B, Prinsen, E, Smets, E & Dessein, S 2011, 'Identification, origin, and evolution of leaf nodulating symbionts of Sericanthe (Rubiaceae)', The Journal of Microbiology, vol. 49, no. 6, pp. 935-941, doi: 10.1007/s12275-011-1163-5. en
dc.identifier.issn 1225-8873 (print)
dc.identifier.issn 1976-3794 (online)
dc.identifier.other 10.1007/s12275-011-1163-5
dc.language.iso en en_US
dc.publisher Springer en_US
dc.rights ⓒ 2011, The Microbiological Society of Korea. The original publication is available at en
dc.subject Burkholderia en
dc.subject Bacterial leaf nodulation en
dc.subject Sericanthe en
dc.subject.lcsh Endosymbiosis en
dc.subject.lcsh Rubiaceae en
dc.subject.lcsh Bacterial diseases of plants -- Identification en
dc.subject.lcsh Leaves -- Diseases and pests-- Identification en
dc.title Identification, origin, and evolution of leaf nodulating symbionts of Sericanthe (Rubiaceae) en
dc.type Postprint Article en

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