Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Show simple item record Van der Nest, Magrieta Aletta Steenkamp, Emma Theodora Slippers, Bernard Mongae, Aobakwe Oratile Van Zyl, Karlien Stenlid, J. Wingfield, Michael J. Wingfield, Brenda D. 2012-02-13T07:23:29Z 2012-02-13T07:23:29Z 2011-11
dc.description.abstract In filamentous fungi, vegetative compatibility among individuals of the same species is determined by the genes encoded at the heterokaryon incompatibility (het) loci. The hyphae of genetically similar individuals that share the same allelic specificities at their het loci are able to fuse and intermingle, while different allelic specificities at the het loci result in cell death of the interacting hyphae. In this study, suppression subtractive hybridization (SSH) followed by pyrosequencing and quantitative reverse transcription PCR were used to identify genes that are selectively expressed when vegetatively incompatible individuals of Amylostereum areolatum interact. The SSH library contained genes associated with various cellular processes, including cell-cell adhesion, stress and defence responses, as well as cell death. Some of the transcripts encoded proteins that were previously implicated in the stress and defence responses associated with vegetative incompatibility. Other transcripts encoded proteins known to be associated with programmed cell death, but have not previously been linked with vegetative incompatibility. Results of this study have considerably increased our knowledge of the processes underlying vegetative incompatibility in Basidiomycetes in general and A. areolatum in particular. en
dc.description.librarian nf2012 en
dc.description.sponsorship The Tree Protection Co-operative Programme (TPCP), the THRIP initiative of the Department of Trade and Industry (DTI) South Africa, as well as the National Research Foundation (NRF). en_US
dc.description.uri en_US
dc.identifier.citation Van Der Nest, MA, Steenkamp, ET, Slippers, B, Mongae, A, Van Zyl, K, Stenlid, J, Wingfield, MJ & Wingfield, BD 2011, 'Gene expression associated with vegetative incompatibility in Amylostereum areolatum', Fungal Genetics and Biology, vol. 48, no. 11, pp. 1034-1043. en
dc.identifier.issn 1087-1845 (print)
dc.identifier.issn 1096-0937 (online)
dc.identifier.other 10.1016/j.fgb.2011.08.001
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2011 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Genetics and Biology, vol. 48, issue 11, 2011, doi : 10.1016/j.fgb.2011.08.001 en
dc.subject Amylostereum areolatum en
dc.subject Vegetative incompatibility en
dc.subject Het loci en
dc.subject Stress and defence responses en
dc.subject Programmed cell death (PCD) en
dc.subject.lcsh Basidiomycetes en
dc.subject.lcsh Cell adhesion en
dc.subject.lcsh Cell death en
dc.subject.lcsh Plant gene expression en
dc.title Gene expression associated with vegetative incompatibility in Amylostereum areolatum en
dc.type Postprint Article en

Files in this item

This item appears in the following Collection(s)

Show simple item record