Detection and genetic analysis of feline immunodeficiency virus (FIVple) in southern African lions (Panthera leo)

Show simple item record Adams, Hayley R. Van Vuuren, Moritz Bosman, Anna-Mari Kania, Stephen Kennedy, Melissa A. 2012-02-01T07:34:11Z 2012-02-01T07:34:11Z 2011-10
dc.description.abstract Feline immunodeficiency virus is a retrovirus of domestic cats that causes immunosuppressive disease and lifelong infection. Lentivirus has also been detected in African lions (Panthera leo). The lentivirus infecting lions in southern Africa has never been isolated; thus, knowledge about its molecular characteristics in these populations is limited. Our investigation used whole blood samples collected opportunistically from free-ranging southern African lions in Kruger National Park, South Africa, and from Hlane Royal National Park in Swaziland to analyse the lentivirus. Whole blood samples from captive exotic felids fromzoos in South Africa and the United States,and fromdomestic cats in South Africa were analysed for comparison. A nested polymerase chain reaction (PCR) assay was used to amplify a portion of the proviral DNA encoding the reverse transcriptase. The nucleotide sequence of all products was determined and examined. The PCR assay was successful in amplifying lion lentivirus, with 52 positive and 65 negative samples. Of the 34 sequences amplified from a variety of felids, six showed an average of 96% homology to domestic feline lentivirus, and 28 showed an average of 94% homology to lion lentivirus. In addition, domestic cat lentivirus nucleic acid was amplified from a captive tiger, demonstrating the possibility of cross-species transmission. en
dc.description.librarian ab2012 en
dc.description.sponsorship Funding was provided by the Center of Excellence in Livestock Diseases and Human Health and the College of Veterinary Medicine, University of Tennessee. Funding for sample collection and storage in South Africa was provided courtesy of the South Africa Veterinary Foundation. en
dc.description.uri en
dc.identifier.citation Adams, HR, Van Vuuren, M, Bosman, AM, Kania, S & Kennedy, MA 2011, 'Detection and genetic analysis of feline immunodeficiency virus (FIVple) in southern African lions (Panthera leo)', South African Journal of Wildlife Research, vol. 41, no. 2, pp. 173-180. en
dc.identifier.issn 0379-4369
dc.identifier.other 7004572625
dc.identifier.other N-9080-2014
dc.language.iso en en
dc.publisher Southern African Wildlife Management Association en
dc.relation.requires Adobe Acrobat Reader en
dc.rights Southern African Wildlife Management Association en
dc.subject Kruger National Park en
dc.subject Lentivirus en
dc.subject Panthera leo en
dc.subject PCR en
dc.subject South Africa en
dc.subject.lcsh Feline immunodeficiency virus en
dc.subject.lcsh Lion en
dc.title Detection and genetic analysis of feline immunodeficiency virus (FIVple) in southern African lions (Panthera leo) en
dc.type Article en

Files in this item

This item appears in the following Collection(s)

Show simple item record