Microsatellite markers for Grosmannia alacris (Ophiostomataceae, Ascomycota) and other species in the G. serpens complex

Duong, Tuan A.; De Beer, Z. Wilhelm; Zanzot, James W.; Wingfield, Michael J.; Wingfield, Brenda D.

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dc.contributor.author	Duong, Tuan A.
dc.contributor.author	De Beer, Z. Wilhelm
dc.contributor.author	Zanzot, James W.
dc.contributor.author	Wingfield, Michael J.
dc.contributor.author	Wingfield, Brenda D.
dc.date.accessioned	2012-01-12T11:58:34Z
dc.date.available	2013-05-31T10:07:45Z
dc.date.issued	2012-05
dc.description.abstract	PREMISE OF THE STUDY: Polymorphic microsatellite markers were developed for the pine infecting fungus, Grosmannia alacris. METHODS AND RESULTS: Sixteen microsatellite markers were developed by using ISSRPCRs and 454 sequencing methods. Seven of these markers showed polymorphisms for a South African population of G. alacris and thirteen markers showed polymorphism when European isolates were included. Most of the primer pairs also amplified four closely related species, G. serpens, Leptographium gibbsii, L. castellanum, and L. yamaokae. CONCLUSION: These new markers will be useful for population studies of G. alacris and other species in the G. serpens complex.	en
dc.description.librarian	nf2012	en
dc.description.sponsorship	Tree Protection and Cooperation Program (TPCP), the National Research Foundation (NRF), the Department of Science and Technology (DST)/NRF, Centre of Excellence in Tree Health Biotechnology (CTHB) and the University of Pretoria, South Africa.	en_US
dc.description.uri	http://www.botany.org/	en_US
dc.identifier.citation	Duong, TA, De Beer, ZW, Zanzot, JW, Wingfield, MJ & Wingfield, BD 2011, 'Microsatellite markers for Grosmannia alacris (Ophiostomataceae, Ascomycota) and other species in the G. serpens complex', American Journal of Botany, vol. 99, no. 5, pp. e216-e219.	en
dc.identifier.issn	0002-9122 (print)
dc.identifier.issn	1537-2197 (online)
dc.identifier.uri	http://hdl.handle.net/2263/17763
dc.language.iso	en	en_US
dc.publisher	Botanical Society of America	en_US
dc.rights	Botanical Society of America. This article is embargoed by the publisher until January 2013.	en
dc.subject	Grosmannia alacris	en
dc.subject.lcsh	Leptographium	en
dc.subject.lcsh	Microsatellites (Genetics)	en
dc.subject.lcsh	Pathogenic microorganisms	en
dc.subject.lcsh	Pine -- Diseases and pests	en
dc.title	Microsatellite markers for Grosmannia alacris (Ophiostomataceae, Ascomycota) and other species in the G. serpens complex	en
dc.type	Postprint Article	en