PREMISE OF THE STUDY: Polymorphic microsatellite markers were developed for the pine
infecting fungus, Grosmannia alacris.
METHODS AND RESULTS: Sixteen microsatellite markers were developed by using ISSRPCRs
and 454 sequencing methods. Seven of these markers showed polymorphisms
for a South African population of G. alacris and thirteen markers showed polymorphism
when European isolates were included. Most of the primer pairs also amplified four
closely related species, G. serpens, Leptographium gibbsii, L. castellanum, and L.
CONCLUSION: These new markers will be useful for population studies of G. alacris and
other species in the G. serpens complex.