Immunohistochemical characterization of lymphocyte and myeloid cell infiltrates in spirocercosis-induced oesophageal nodules

Show simple item record

dc.contributor.author Dvir, Eran
dc.contributor.author Schoeman, Johan P.
dc.contributor.author Clift, Sarah J.
dc.contributor.author McNeilly, T.N.
dc.contributor.author Mellanby, R.J.
dc.date.accessioned 2011-10-27T08:09:37Z
dc.date.available 2012-10-31T00:20:03Z
dc.date.issued 2011-10
dc.description.abstract Spirocerca lupi is a nematode that infects the dog’s oesophagus and promotes the formation of an inflammatory fibroblastic nodule that progresses to sarcoma in approximately 25% of cases. Spirocercosis-associated oesophageal sarcoma is an excellent and under-utilized spontaneous model of parasite-associated malignancy. The inflammatory infiltrate of paraffin-embedded, non-neoplastic oesophageal nodules (n = 46), neoplastic nodules (n = 25) and normal oesophagus (n = 14) was examined by immunohistochemistry using MAC387 (myeloid cells), CD3 (T cells), Pax5 (B cells) and FoxP3 (T regulatory cells) antibodies. Myeloid cells predominated in 70% of nodules, in pockets around the worms’ migratory tracts and in necro-ulcerative areas in neoplastic cases. T cells predominated in 23% of cases with a focal or diffuse distribution, in the nodule periphery. No significant differences were observed between neoplastic and non-neoplastic stages. FoxP3+ cells were observed in low numbers, not significantly different from the controls. The inflammation in spirocercosis is characterized by pockets of pus surrounded by organized lymphoid foci. There was no evidence of a local accumulation of FoxP3+ cells, unlike many previous studies that have reported an increase in FoxP3+ T cells in both malignancies and parasite infections. The triggering factor(s) driving the malignant transformation of the spirocercosis-associated chronic inflammatory nodule warrants further investigation. en
dc.description.sponsorship This study was funded by Petplan Charitable Trust. en
dc.description.uri http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3024 en
dc.identifier.citation Dvir, E, Schoeman, JP, Clift, SJ, McNeilly, TN & Mellanby, R 2011, 'Immunohistochemical characterization of lymphocyte and myeloid cell infiltrates in spirocercosis-induced oesophageal nodules', Parasite Immunology, vol. 33, no. 10, pp. 545-553. en
dc.identifier.issn 0141-9838 (print)
dc.identifier.issn 1365-3024 (online)
dc.identifier.other 10.1111/j.1365-3024.2011.01316.x
dc.identifier.other 7005814117
dc.identifier.other 7004445917
dc.identifier.other J-6765-2013
dc.identifier.uri http://hdl.handle.net/2263/17476
dc.language.iso en en
dc.publisher Wiley-Blackwell en
dc.rights © 2011 Blackwell Publishing Ltd. The definite version is available at http://onlinelibrary.wiley.com/. This article is embargoed by the publisher until October 2012. en
dc.subject CD3 en
dc.subject FoxP3 en
dc.subject MAC387 en
dc.subject Pax5 en
dc.subject Sarcoma en
dc.subject Spirocerca lupi en
dc.subject T regulatory cells en
dc.subject Nematodes as carriers of disease en
dc.subject.lcsh Veterinary clinical pathology en
dc.subject.lcsh Veterinary medicine -- Diagnosis en
dc.subject.lcsh Nematodes en
dc.title Immunohistochemical characterization of lymphocyte and myeloid cell infiltrates in spirocercosis-induced oesophageal nodules en
dc.type Postprint Article en


Files in this item

This item appears in the following Collection(s)

Show simple item record