BACKGROUND: The LightCyclerH Mycobacterium Detection Kit based on real-time PCR technology for the detection of Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii was recently developed. This study evaluated its analytical sensitivity, specificity and reproducibility. METHODOLOGY/PRINCIPAL FINDINGS: Plasmid standards were prepared and used to determine the limit of detection. The assay was also performed against organisms other than mycobacteria, other mycobacterial strains and interfering substances to exclude cross-reactivity and interference. Reference standards were prepared and tested to assess the assay’s reproducibility. All PCR assays were performed using the LightCyclerH 2.0 Instrument. The detection limit for M. tuberculosis was 28 copies per microlitre. Neither cross-reactivity nor interference occurred with non-mycobacterial organisms and substances tested. Overall reproducibility for consecutive measurements, run-to-run, lot-to-lot, day-to-day and laboratory-to-laboratory achieved a coefficient of variance of less than two percent. SIGNIFICANCE: The LightCyclerH Mycobacterium Detection kit has shown to be a robust and accurate assay with the potential to be used as a rapid TB diagnostic test.
Gcebe, Nomakorinte; Rutten, Victor P.M.G.; Gey van Pittius, Nicolaas C.; Michel, Anita Luise(Wiley-Blackwell, 2013-11)
It has been hypothesized that a variety of non-tuberculous mycobacteria (NTM) species to which livestock and wildlife species are naturally exposed induce broadly cross-reactive anti-mycobacterial immune responses which ...
Mbugi, Erasto V.; Katale, Bugwesa Z.; Streicher, Elizabeth M.; Keyyu, Julius D.; Kendall, Sharon L.; Dockrell, Hazel M.; Michel, Anita Luise; Rweyemamu, Mark M.; Warren, Robin M.; Matee, Mecky I.; Van Helden, Paul D.; Couvin, David; Rastogi, Nalin(Public Library of Science, 2016-05-05)
The aim of this study was to assess and characterize Mycobacterium tuberculosis complex
(MTBC) genotypic diversity in Tanzania, as well as in neighbouring East and other several
African countries. We used spoligotyping ...
Michel, Anita Luise(International Office of Epizootics, 2014)
Early discoveries in the field of tuberculosis more than a century ago indicated
that the success of disease control in human populations would depend on the
success of control measures in animals, and vice versa. ...