Browsing Research Articles (Plant Science) by UP Author "Vorster, Barend Juan"

Browsing Research Articles (Plant Science) by UP Author "Vorster, Barend Juan"

Sort by: Order: Results:

  • Agroinfiltration contributes to VP1 recombinant protein degradation 
    Pillay, Priyen; Kunert, Karl J.; Van Wyk, Stefan George; Makgopa, Matome Eugene; Cullis, C.A. (Christoper Ashley); Vorster, Barend Juan (Routledge, 2016-08)
    There is a growing interest in applying tobacco agroinfiltration for recombinant protein production in a plant based system. However, in such a system, the action of proteases might compromise recombinant protein ...
  • Cysteine protease and cystatin expression and activity during soybean nodule development and senescence 
    Van Wyk, Stefan George; Du Plessis, Magdeleen; Cullis, C.A. (Christoper Ashley); Kunert, Karl J.; Vorster, Barend Juan (BioMed Central, 2014)
    BACKGROUND : Nodules play an important role in fixing atmospheric nitrogen for soybean growth. Premature senescence of nodules can negatively impact on nitrogen availability for plant growth and, as such, we need a better ...
  • Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus 
    Kiggundu, Andrew; Muchwezi, Josephine; Van der Vyver, Christell; Viljoen, Altus; Vorster, Barend Juan; Schluter, Urte; Kunert, Karl J.; Michaud, Dominique (Wiley, 2010-02)
    The general potential of plant cystatins for the development of insect-resistant transgenic plants still remains to be established given the natural ability of several insects to compensate for the loss of active cysteine ...
  • Drought stress responses in soybean roots and nodules 
    Kunert, Karl J.; Vorster, Barend Juan; Fenta, Berhanu A.; Kibido, Tsholofelo Reineth; Dionisio, Giuseppe; Foyer, Christine H. (Frontiers Research Foundation, 2016-07-12)
    Drought is considered to be a major threat to soybean production worldwide and yet our current understanding of the effects of drought on soybean productively is largely based on studies on above-ground traits. Although ...
  • Microsatellite association with seed protein content and flowering time in Nigerian cowpea cultivars 
    Afiukwa, Celestine A.; Kunert, Karl J.; Vorster, Barend Juan; Cullis, C.A. (Christoper Ashley); Ubi, Benjamin E. (Academic Journals, 2011-12-12)
    Cowpea microsatellites were evaluated for their potential to be used as markers associated with seed protein content and plant flowering time. Seeds with high and low protein content as well as plants with early and late ...
  • Potential use of phytocystatins in crop improvement, with a particular focus on legumes 
    Kunert, Karl J.; Van Wyk, Stefan George; Cullis, C.A. (Christoper Ashley); Vorster, Barend Juan; Foyer, Christine H. (Oxford University Press, 2015-06)
    Phytocystatins are a well-characterized class of naturally-occurring protease inhibitors that function by preventing the catalysis of papain-like cysteine proteases. The action of cystatins in biotic stress resistance ...
  • Proteolysis of recombinant proteins in bioengineered plant cells 
    Pillay, Priyen; Schluter, Urte; Van Wyk, Stefan; Kunert, Karl J.; Vorster, Barend Juan (Routledge, 2014-05)
    Plants are increasingly used as alternative expression hosts for the production of recombinant proteins offering many advantages including higher biomass and the ability to perform posttranslational modifications on ...
  • Review : The future of cystatin engineering 
    Van Wyk, Stefan George; Kunert, Karl J.; Cullis, C.A. (Christoper Ashley); Pillay, Priyen; Makgopa, Matome Eugene; Schluter, Urte; Vorster, Barend Juan (Elsevier, 2016-05)
    Plant cystatins are naturally occurring protease inhibitors that prevent proteolysis by papain-like cysteine proteases. Their protective action against environmental stresses has been relatively well characterised. Still, ...
  • Transfer of genetic material between the chloroplast and nucleus : how is it related to stress in plants? 
    Cullis, C.A. (Christoper Ashley); Vorster, Barend Juan; Van der Vyver, Christell; Kunert, Karl J. (Oxford University Press, 2008)
    BACKGROUND: The presence of chloroplast-related DNA sequences in the nuclear genome is generally regarded as a relic of the process by which genes have been transferred from the chloroplast to the nucleus. The remaining ...
  • Use of transgenic Oryzacystatin-I expressing plants enhances recombinant protein production 
    Pillay, Priyen; Kibido, Tsholofelo Reineth; Du Plessis, M.; Van der Vyver, Christell; Beyene, Getu; Vorster, Barend Juan; Kunert, Karl J.; Schluter, Urte (Springer, 2012-09-11)
    Plants are an effective and inexpensive host for the production of commercially interesting heterologous recombinant proteins. The Escherichia coli-derived glutathione reductase was transiently expressed as a recombinant ...