Computer-based audiometry allows for novel applications, including remote testing and automation, which may improve the accessibility and efficiency of hearing assessment in various clinical and occupational health settings. This study describes the validity of computer-based diagnostic air and forehead bone conduction audiometry when compared to conventional industry standard audiometry in a soundbooth environment. A sample of 30 subjects (19 to 77 years of age) was assessed with a computer-based (KUDUwave 5000) and industry standard conventional audiometer (GSI 61) to compare air and bone conduction thresholds and test-retest reliability. Air conduction thresholds for the two audiometers corresponded within 5dB or less in more than 90% of instances with an average absolute difference of 3.5 dB (3.8 SD) and a 95% confidence interval of 2.6 to 4.5 dB. Bone conduction thresholds for the two audiometers corresponded within 10 dB or less in 92% of instances with an average absolute difference of 4.9 dB (4.9 SD) and a 95% confidence interval of 3.6 to 6.1 dB. The average absolute test-retest threshold difference for bone conduction on the industry standard audiometer was 5.1 dB (5.3 SD) and for the computer-based audiometer 7.1 dB (6.4 SD). Computer-based audiometry provided air and bone conduction thresholds within the test-retest reliability limits of industry standard audiometry.