Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species

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dc.contributor.author Beloqui, Ana
dc.contributor.author Nechitaylo, Taras Y.
dc.contributor.author Lopez-Cortes, Nieves
dc.contributor.author Ghazi, Azam
dc.contributor.author Guazzaroni, María-Eugenia
dc.contributor.author Polaina, Julio
dc.contributor.author Strittmatter, Axel W.
dc.contributor.author Reva, Oleg N.
dc.contributor.author Waliczek, Agnes
dc.contributor.author Yakimov, Michail M.
dc.contributor.author Golyshina, Olga V.
dc.contributor.author Ferrer, Manuel
dc.contributor.author Golyshin, Peter N.
dc.date.accessioned 2011-03-23T06:53:25Z
dc.date.available 2011-03-23T06:53:25Z
dc.date.issued 2010-09
dc.description.abstract The guts and casts of earthworms contain microbial assemblages that process large amounts of organic polymeric substrates from plant litter and soil; however, the enzymatic potential of these microbial communities remains largely unexplored. In the present work, we retrieved carbohydrate-modifying enzymes through the activity screening of metagenomic fosmid libraries from cellulose-depleting microbial communities established with the fresh casts of two earthworm species, Aporrectodea caliginosa and Lumbricus terrestris, as inocula. Eight glycosyl hydrolases (GHs) from the A. caliginosa-derived community were multidomain endo- -glucanases, -glucosidases, - cellobiohydrolases, -galactosidase, and -xylosidases of known GH families. In contrast, two GHs derived from the L. terrestris microbiome had no similarity to any known GHs and represented two novel families of -galactosidases/ -arabinopyranosidases. Members of these families were annotated in public databases as conserved hypothetical proteins, with one being structurally related to isomerases/dehydratases. This study provides insight into their biochemistry, domain structures, and activesite architecture. The two communities were similar in bacterial composition but significantly different with regard to their eukaryotic inhabitants. Further sequence analysis of fosmids and plasmids bearing the GH-encoding genes, along with oligonucleotide usage pattern analysis, suggested that those apparently originated from Gammaproteobacteria (pseudomonads and Cellvibrio-like organisms), Betaproteobacteria (Comamonadaceae), and Alphaproteobacteria (Rhizobiales). en
dc.identifier.citation Beloqui, A, Nechitaylo, TY, Lopez-Cortes, N, Ghazi, A, Guazzaroni, ME, Polaina, J, Strittmatter, AW, Reva, O, Waliczek, A, Yakimov, MM, Golyshina, OV, Ferrer, M & Golyshin, PN 2010, 'Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species', Applied and Environmental Microbiology, vol. 76, no. 17, pp. 5934–5946. [http://aem.asm.org/] en
dc.identifier.issn 0099-2240
dc.identifier.other 10.1128/AEM.00902-10
dc.identifier.uri http://hdl.handle.net/2263/16102
dc.language.iso en en_US
dc.publisher American Society for Microbiology en_US
dc.rights © 2010, American Society for Microbiology. All Rights Reserved. en_US
dc.subject Glycosyl hydrolases en
dc.subject Earthworm casts analysis en
dc.subject Microbial communities en
dc.subject.lcsh Enzymatic analysis en
dc.title Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species en
dc.type Article en


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