dc.contributor.author |
Mavundza, Edison Johannes
|
|
dc.contributor.author |
Tshikalange, Thilivhali Emmanuel
|
|
dc.contributor.author |
Lall, Namrita
|
|
dc.contributor.author |
Hussein, Ahmed A.
|
|
dc.contributor.author |
Mudau, Fhatuwani Nixwell
|
|
dc.contributor.author |
Meyer, Jacobus Johannes Marion
|
|
dc.date.accessioned |
2011-02-07T06:37:35Z |
|
dc.date.available |
2011-02-07T06:37:35Z |
|
dc.date.issued |
2010-12 |
|
dc.description.abstract |
Bioassay-guided fractionation of an ethanolic extract from aerial parts of Athrixia phylicoides using silica and sephadex column chromatography led to the isolation of three flavonoids. The compounds were identified as: 5-hydroxy-6,7,8,3’,4’,5’-hexamethoxyflavon-3-ol (1), 3-0-demethyldigicitrin (2), and Quecertin (3). Isolated compounds together with ethanol crude extract were tested for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-spectrophotometric assay, while cytotoxicity effect was determined using XTT colorimetric assay. The crude extract showed a concentration-dependent radical scavenging activity with EC50 value of 10.64 ± 0.08 μg/ml. Compound 3 was the most potent radical scavenger, exhibiting EC50 value of 1.27 ± 0.25 μg/ml, followed by compound 1 and 2 showing 2.74 ± 0.10 and 3.41 ± 0.09 μg/ml respectively. The crude extract showed no or little toxicity on Vero cells at lower concentrations tested exhibiting the IC50 value of 107.8 ± 0.13 μg/ml. Compound 3 showed minimal toxicity effect by exhibiting IC50 value of 81.38 ± 0.33 μg/ml as compared to compound 2 (IC50,
28.92 ± 0.12 μg/ml) and compound 1 (IC50, 27.91 ± 0.18 μg/ml). The results obtained from this study
provide a clear rationale for the medicinal uses of A. phylicoides. |
en |
dc.identifier.citation |
Mavundza, EJ, Tshikalange, TE, Lall, N, Hussein, AA, Mudau, FN & Meyer, JJM 2010, 'Antioxidant activity and cytotoxicity effect of flavonoids isolated from athrixia phylicoides', Journal of Medicinal Plant Research, vol. 4, no. 23, pp. 2584-2587. [www.academicjournals.org/JMPR] |
en |
dc.identifier.issn |
1996-0875 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/15828 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Academic Journals |
en_US |
dc.rights |
© 2010 Academic Journals |
en_US |
dc.subject |
Athrixia phylicoides |
en |
dc.subject |
Antioxidant activity |
en |
dc.subject |
Cytotoxicity |
en |
dc.subject |
2,2-Diphenyl-1-picrylhydrazyl |
en |
dc.subject |
Vero cells |
en |
dc.subject.lcsh |
Flavonoids |
en |
dc.title |
Antioxidant activity and cytotoxicity effect of flavonoids isolated from athrixia phylicoides |
en |
dc.type |
Article |
en |