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dc.contributor.author | Mavundza, Edison Johannes![]() |
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dc.contributor.author | Tshikalange, Thilivhali Emmanuel![]() |
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dc.contributor.author | Lall, Namrita![]() |
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dc.contributor.author | Hussein, Ahmed A.![]() |
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dc.contributor.author | Mudau, Fhatuwani Nixwell![]() |
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dc.contributor.author | Meyer, Jacobus Johannes Marion![]() |
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dc.date.accessioned | 2011-02-07T06:37:35Z | |
dc.date.available | 2011-02-07T06:37:35Z | |
dc.date.issued | 2010-12 | |
dc.description.abstract | Bioassay-guided fractionation of an ethanolic extract from aerial parts of Athrixia phylicoides using silica and sephadex column chromatography led to the isolation of three flavonoids. The compounds were identified as: 5-hydroxy-6,7,8,3’,4’,5’-hexamethoxyflavon-3-ol (1), 3-0-demethyldigicitrin (2), and Quecertin (3). Isolated compounds together with ethanol crude extract were tested for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-spectrophotometric assay, while cytotoxicity effect was determined using XTT colorimetric assay. The crude extract showed a concentration-dependent radical scavenging activity with EC50 value of 10.64 ± 0.08 μg/ml. Compound 3 was the most potent radical scavenger, exhibiting EC50 value of 1.27 ± 0.25 μg/ml, followed by compound 1 and 2 showing 2.74 ± 0.10 and 3.41 ± 0.09 μg/ml respectively. The crude extract showed no or little toxicity on Vero cells at lower concentrations tested exhibiting the IC50 value of 107.8 ± 0.13 μg/ml. Compound 3 showed minimal toxicity effect by exhibiting IC50 value of 81.38 ± 0.33 μg/ml as compared to compound 2 (IC50, 28.92 ± 0.12 μg/ml) and compound 1 (IC50, 27.91 ± 0.18 μg/ml). The results obtained from this study provide a clear rationale for the medicinal uses of A. phylicoides. | en |
dc.identifier.citation | Mavundza, EJ, Tshikalange, TE, Lall, N, Hussein, AA, Mudau, FN & Meyer, JJM 2010, 'Antioxidant activity and cytotoxicity effect of flavonoids isolated from athrixia phylicoides', Journal of Medicinal Plant Research, vol. 4, no. 23, pp. 2584-2587. [www.academicjournals.org/JMPR] | en |
dc.identifier.issn | 1996-0875 | |
dc.identifier.uri | http://hdl.handle.net/2263/15828 | |
dc.language.iso | en | en_US |
dc.publisher | Academic Journals | en_US |
dc.rights | © 2010 Academic Journals | en_US |
dc.subject | Athrixia phylicoides | en |
dc.subject | Antioxidant activity | en |
dc.subject | Cytotoxicity | en |
dc.subject | 2,2-Diphenyl-1-picrylhydrazyl | en |
dc.subject | Vero cells | en |
dc.subject.lcsh | Flavonoids | en |
dc.title | Antioxidant activity and cytotoxicity effect of flavonoids isolated from athrixia phylicoides | en |
dc.type | Article | en |