Effect of heterologous seminal plasma and semen extenders on motility of frozen–thawed ram spermatozoa

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dc.contributor.author Mataveia, G.A.
dc.contributor.author Terblanche, S.J.
dc.contributor.author Nothling, Johan O.
dc.contributor.author Gerber, David
dc.date.accessioned 2010-10-27T07:23:56Z
dc.date.available 2010-10-27T07:23:56Z
dc.date.issued 2010
dc.description.abstract Ram seminal plasma increases the fertility of frozen–thawed ram spermatozoa deposited into the cervix. The aim of the current study was to compare the effect of ram seminal plasma to that of bull seminal plasma, dog prostatic fluid, protein-free TALP, TrilEq (Triladyl with 0.5 m of Equex STM paste added to each 100 m ) and heat-treated skim milk on longevity and percentages of progressively motile and aberrantly motile frozen–thawed ram spermatozoa. Three ejaculates from each of 6 rams were extended in TrilEq, pooled and frozen in straws as a single batch per ram. One hundred and eight straws (3 straws from each ram for each fluid) were thawed in random order. Once thawed, a straw was emptied into a tube with 0.85m of the appropriate fluid at 37 °C and kept at that temperature for 6 h. Motility was assessed at ×200 magnification immediately (time zero) and 2, 4 and 6 h after thawing. Progressive motility decreased from each time to the next (P < 0.05) and was 39.0%(0 h), 26.0%(2 h), 19.6%(4 h) and 12.6%(6 h);SEM1.24, n=108 for each group.Ram seminal plasma resulted in higher progressive motility than bull seminal plasma, lower than milk, and similar to the other fluids. Ram seminal plasma resulted in lower aberrant motility than protein-free TALP and similar aberrant motility to other fluids. The effect of ram seminal plasma and dog prostatic fluid was very similar. The effect of ram seminal plasma on the fertility of frozen–thawed ram spermatozoa deposited into the cervix is not due an exceptionally beneficial effect on the motility of spermatozoa. en
dc.identifier.citation Mataveia, GA, Terblanche, SJ, Nothling, OJ & Gerber, D 2010, 'Effect of heterologous seminal plasma and semen extenders on motility of frozen–thawed ram spermatozoa', Journal of the South African Veterinary Association, vol. 81, no. 3, pp. 139–142. [http://www.journals.co.za/ej/ejour_savet.html] en
dc.identifier.issn 0038-2809
dc.identifier.other 6602528464
dc.identifier.other O-6382-2014
dc.identifier.uri http://hdl.handle.net/2263/15079
dc.language.iso en en
dc.publisher South African Veterinary Association en
dc.relation.requires Adobe Acrobat Reader en
dc.rights South African Veterinary Association en
dc.subject Frozen-thawed semen en
dc.subject Ram spermatozoa en
dc.subject Seminal plasma sperm en
dc.subject.lcsh Frozen semen en
dc.subject.lcsh Spermatozoa -- Motility en
dc.title Effect of heterologous seminal plasma and semen extenders on motility of frozen–thawed ram spermatozoa en
dc.type Article en


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