Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis

Show simple item record McClusky, Leon M. Patrick, Sean Mark Barnhoorn, Irene E.J. Van Dyk, Jacobus C. De Jager, Christiaan Bornman, Maria S. (Riana) 2010-08-23T08:03:53Z 2010-08-23T08:03:53Z 2009-08
dc.description.abstract In a previous study on the effects of gestational and lactational exposure of para-nonylphenol on male rats, we noted in both induced and uninduced rats, that variations in cleaved caspase- 3 immunostaining patterns were associated with distinct nuclear alterations in mainly basally located germ cells (spermatogonia and preleptotene spermatocytes). These were re-analysed and compared with cleaved caspase-3-labeled germ cells in the aging human and the spermatogenically active catfish testis. In the rat testes, cytoplasmic immunostaining was progressively associated with lateral compression of the nucleus, its break up into large pieces which can contain immunostained marginated chromatin masses. The pale remnants of the nucleus continued to shrink in size concomitant with the appearance of blue-purplish stained regions in the cytoplasm similar in color to the condensed chromatin in spermatids, a condition which was TUNEL-negative. These large clumps of chromatin also eventually disappeared, giving rise to cells resembling cytoplasmic ghosts, a condition which was TUNEL-positive. By contrast, the immunolabeled nuclei of human and catfish germ cells condensed into a single mass, after which they lost immunoreactivity. To exclude the possibility that these observations could reflect alterations in Sertoli nuclei, rat testicular sections were probed with a mouse anti-human GATA-4 monoclonal (MHM) antibody. The MHM was, however, the second of two GATA-4 antibodies tested, with a goat anti-mouse polyclonal (GMP) initially used to label rat the Sertoli nuclei. GMP unexpectedly, but distinctly labeled the complete development of the acrosome in the rat testis, a fortuitous finding with utility for staging of the seminiferous epithelium. en_US
dc.identifier.citation McClusky, LM, Patrick, S, Barnhoorn, IEJ, Van Dyk, JC, De Jager, C & Bornman, MS 2009, 'Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis', Journal of Molecular Histology, vol. 40, no. 4, pp. 287-299. [] en_US
dc.identifier.issn 1567-2379
dc.identifier.other 10.1007/s10735-009-9240-3
dc.language.iso en en_US
dc.publisher Springer en_US
dc.rights Springer en_US
dc.subject Apoptosis en_US
dc.subject Germ cells en_US
dc.subject Nucleus en_US
dc.subject Cleaved caspase-3 en_US
dc.subject Acrosome en_US
dc.subject GATA-4 en_US
dc.subject Immunohistochemistry en_US
dc.title Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis en_US
dc.type Postprint Article en_US

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