The polymerase chain reaction was used to detect trypanosomes in samples collected from cattle, wild animals and tsetse
flies in KwaZulu-Natal Province, South Africa. A total of 673 samples from cattle and 266 from tsetse flies in the study area
located near the Hluhluwe-Umfolozi Game Reserve were analysed. Both Trypanosoma congolense and T. vivax were found
as single or mixed infections in cattle and tsetse flies. Moreover, the T. congolense in the infections were found to comprise 2
genotypic groups: the Savannah-type and the Kilifi-type, which were present either as single or mixed infections in cattle
and in tsetse flies.