Section 5 describes experiments to isolate the toxicogenic saprophyte. These experiments showed that an organism of the Clostritium type was isolated from material which produced lamsiekte when given to susceptible animals per os, or by inoculation subcutaneously, or by other routes. This organism was tentatively named Clostridium parabotulinum bovis. This organism produced in culture media an exotoxin which in unfiltered material or after filtration through a Berkefeld candle, produced typical symptoms of lamsiekte in susceptible animals.
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