Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases

Show simple item record Arrebola, Eva Sivakumar, Dharini Bacigalupo, Romina Korsten, Lise 2009-10-28T06:14:31Z 2009-10-28T06:14:31Z 2009
dc.description.abstract Bacillus amyloliquefaciens PPCB004 was selected as a potential antagonist to control Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer on peach fruit. The HPLC data of PPCB004 indicated the lipopeptides iturin A, fengycin and surfactin as secondary metabolites. The GC/MS analysis of PPCB004 showed 3-hydroxy-2-butanone as the dominant compound (97.52% of relative peak area). Thyme (TO) and lemongrass (LO) oils showed over 50% and 25% inhibition of radial mycelial growth respectively with 8 μl oil per plate for all pathogens. Combination treatment with both oils failed to increase the percentage inhibition of radial mycelial growth of the pathogens. Combined application of PPCB004 with TO or LO was tested to assess the effectiveness in the control of these pathogens during postharvest storage. The biofilm formation of PPCB004 was significantly higher in LO than TO. LO (6 μl plate-1) and PPCB004 completely inhibited the mycelial growth of the pathogens. Fruit inoculation trials with PPCB004 þ LO in NatureFlexTM modified atmosphere packaging (MAP), showed lower disease incidence and severity at 25°C for 5 d than treatments with PPCB004 + MAP, PPCB004 + TOþMAP, LO + MAP, TO + MAP or stand-alone MAP. On naturally infected fruit, PPCB004 + LO + MAP and LO + MAP treatments retained the total soluble solids/titratable acidity ratio and flesh firmness but failed to stimulate the levels of total phenolic content, phenylalanine ammonia-lyase, β-1,3-glucanase and chitinase activities. Combination of PPCB004 (spray treatment) + LO (in pad delivery system) in NatureFlexTM MAP showed absence of disease and off-flavour development, retained the overall appearance and increased the overall acceptance at market shelf conditions (20°C for 2 d) after cold storage at 4°C for 14 d. en_US
dc.identifier.citation Arrebola, E., et al., Combined application of antagonist Bacillus amyloliquefaciens and..., Crop Protection (2009), doi:10.1016/j.cropro.2009.08.001 en_US
dc.identifier.issn 0261-2194
dc.identifier.other 10.1016/j.cropro.2009.08.001
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights Elsevier en_US
dc.subject Prunus persica (L.) Batsch en
dc.subject Fungal pathogens en
dc.subject Biocontrol agent en
dc.subject Essential oil en
dc.subject.lcsh Peach -- Diseases and pests en
dc.subject.lcsh Fungal diseases of plants en
dc.subject.lcsh Pathogenic microorganisms en
dc.subject.lcsh Biological pest control agents en
dc.subject.lcsh Essences and essential oils en
dc.subject.lcsh Bacillus (Bacteria) en
dc.title Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases en_US
dc.type Postprint Article en_US

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