Laboratory-reared Aedes aegypti (Linnaeus, 1762), Culex pipiens fatigans Wiedemann, 1828, and trap-caught Culicoides biting midges were fed on African horsesickness (AHS) virus solutions and on horses infected with AHS. Attempts to isolate AHS virus from these insects from 1 to 40 days after feeding by intracerebral inoculation of suckling mice were unsuccessful. The successful artificial infection of mosquitoes with AHS virus and biological transmission of AHS virus by Culicoides spp. recorded by other workers could not be repeated. Multiplication of AHS virus in these insects will have to be shown before existing claims of successful biological transmission can be accepted completely.
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