Abstract:
Listeria monocytogenes is a ubiquitous bacterial pathogen that threatens the food chain
and human health. In this study, whole-genome sequencing (WGS) was used for the genomic
characterization of L. monocytogenes (n = 24) from beef and beef-based products. Multilocus Sequence
Type (MLST) analysis revealed that ST204 of CC204 was the most common sequence type (ST). Other
sequence types detected included ST1 and ST876 of CC1, ST5 of CC5, ST9 of CC9, ST88 of CC88, ST2
and ST1430 of CC2, and ST321 of CC321. Genes encoding for virulence factors included complete
LIPI-1 (pfrA-hly-plcA-plcB-mpl-actA) from 54% (13/24) of the isolates of ST204, ST321, ST1430, and
ST9 and internalin genes inlABC that were present in all the STs. All the L. monocytogenes STs carried
four intrinsic/natural resistance genes, fosX, lin, norB, and mprF, conferring resistance to fosfomycin,
lincosamide, quinolones, and cationic peptides, respectively. Plasmids pLGUG1 and J1776 were
the most detected (54% each), followed by pLI100 (13%) and pLM5578 (7%). The prophage profile,
vB_LmoS_188, was overrepresented amongst the isolates, followed by LP_101, LmoS_293_028989,
LP_030_2_021539, A006, and LP_HM00113468. Listeria genomic island 2 (LGI-2) was found to be
present in all the isolates, while Listeria genomic island 3 (LGI-3) was present in a subset of isolates
(25%). The type VII secretion system was found in 42% of the isolates, and sortase A was present
in all L. monocytogenes genomes. Mobile genetic elements and genomic islands did not harbor any
virulence, resistance, or environmental adaptation genes that may benefit L. monocytogenes. All the STs
did not carry genes that confer resistance to first-line antibiotics used for the treatment of listeriosis.
The characterization of L. monocytogenes in our study highlighted the environmental resistance and
virulence potential of L. monocytogenes and the risk posed to the public, as this bacterium is frequently
found in food and food processing environments.
