Browsing by UP Author "Zweygarth, Erich"

Browsing by UP Author "Zweygarth, Erich"

Sort by: Order: Results:

  • Zweygarth, Erich; Josemans, A.I.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2001)
    Chemically defined media, termed SFMC-23 and SFMC-36, were devised for the in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants. Both media were based on Dulbecco's ...
  • Zweygarth, Erich; Josemans, A.I.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2001)
    The Welgevonden stock of Cowdria ruminantium, aetiologic agent of heartwater, was continuously propagated in DH82 cells, a continuous canine macrophage-monocyte cell line. Cultures of DH82 cells were readily ...
  • Dugat, Thibaud; Rossignol, Marie-Noelle; Rue, Olivier; Loux, Valentin; Marthey, Sylvain; Moroldo, Marco; Silaghi, Cornelia; Hoper, Dirk; Frohlich, Julia; Zweygarth, Erich; Lagree, Anne-Claire; Boulouis, Henri-Jean; Haddad, Nadia (American Society for Microbiology, 2016-11-01)
    Anaplasma phagocytophilum is a zoonotic tick-borne intracellular bacterium responsible for granulocytic anaplasmosis. As it is difficult to isolate and cultivate, only 20 A. phagocytophilum genomes have been sequenced to ...
  • Diaz-Sanchez, Sandra; Hernandez-Jarguin, Angelica; Fernandez de Mera, Isabel G.; Alberdi, Pilar; Zweygarth, Erich; Gortazar, Christian; De la Fuente, Jose (American Society for Microbiology, 2018-02-01)
    Here, we report the draft genome sequences of isolates of Anaplasma phagocytophilum, Anaplasma marginale, and Anaplasma ovis. The genomes of A. phagocytophilum (human), A. marginale (cattle), and A. ovis (goat) isolates ...
  • Cabezas-Cruz, Alejandro; Zweygarth, Erich; Vancova, Marie; Broniszewska, Marzena; Grubhoffer, Libor; Passos, Lygia Maria Friche; Ribeiro, Mucio Flavio Barbosa; Alberdi, Pilar; De la Fuente, Jose (Society for General Microbiology, 2016-03)
    Recently, we obtained a rickettsial isolate (Ehrlichia sp. UFMG-EVT) from the haemolymph of engorged Rhipicephalus microplus tick females. On the basis of maximum-likelihood phylogenetic analysis using 16S rRNA gene, groEL, ...
  • Zweygarth, Erich; Kaminsky, R.; Moloo, S.K.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1994)
    Two Trypanosoma vivax stocks were initiated in culture with tsetse or culture-derived metacyclics. They were propagated axenically as bloodstream trypomastigotes at 35°C in 4% C0₂ in air. Populations of trypanosomes were ...
  • Ferrolho, Joana; Simpson, Jennifer; Hawes, Philippa; Zweygarth, Erich; Bell-Sakyi, Lesley (Elsevier, 2016-06)
    Canine monocytic ehrlichiosis is caused by Ehrlichia canis, a small gram-negative coccoid bacterium that infects circulating monocytes. The disease is transmitted by the brown dog tick Rhipicephalus sanguineus s.l. and is ...
  • Oosthuizen, Marinda C.; Zweygarth, Erich; Collins, Nicola E.; Troskie, Milana; Penzhorn, Barend Louis (American Society for Microbiology, 2008-07)
    Babesiosis in a sable antelope (Hippotragus niger Harris, 1838) was first reported in 1930; the parasite was named Babesia irvinesmithi. Recently, specimens from an adult sable that presented with a sudden onset of disease ...
  • Zweygarth, Erich; Van Niekerk, C.; Just, M.C.; De Waal, D.T.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)
    A South African Babesia sp. of cattle which is as yet unclassified, was continuously cultivated in micro-aerophilous stationary-phase culture. The parasites were resuscitated from a blood stabilate stored in liquid nitrogen. ...
  • Zweygarth, Erich; Just, M.C.; De Waal, D.T.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1997)
    By means of an in vitro culture technique, 75 samples of horse blood were examined for Babesia equi, a causative agent of equine piroplasmosis. At the time of culture initiation, 15 samples were microscopically positive ...
  • Van Niekerk, C.J.; Zweygarth, Erich; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1996)
    Babesia occultans, the causative agent of a benign form of cattle babesiosis in South Africa, was continuously cultivated in microaerophilous stationary-phase culture. A modified medium, 199, supplemented with either ...
  • Zweygarth, Erich; Josemans, A.I.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2003)
    The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The ...
  • Zweygarth, Erich; Meyer, P.; Lopez-Rebollar, Laura M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Twenty blood samples of zebras ( Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier ...
  • Liebenberg, Junita; Steyn, Helena C.; Josemans, Antoinette I.; Faber, Erika; Zweygarth, Erich (AOSIS OpenJournals, 2020-06-24)
    Three isolates of Ehrlichia ruminantium (Kümm 2, Omatjenne and Riverside), the causative agent of heartwater in domestic ruminants, were isolated in Ixodes scapularis (IDE8) tick cell cultures using the leukocyte fraction ...
  • Alberdi, Pilar; Ayllón, Nieves; Cabezas-Cruz, Alejandro; Bell-Sakyi, Lesley; Zweygarth, Erich; Stuen, Snorre; De la Fuente, José (Elsevier, 2015-09)
    Anaplasma phagocytophilum is an intracellular rickettsial pathogen transmitted by Ixodes spp. ticks, which causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever (TBF) in ruminants. In the United ...
  • Zweygarth, Erich; Van Heerden, Henriette; Allsopp, Basil A.; Josemans, A.I.; Van Strijp, M.F.; Allsopp, M.T.E.P.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An effective culture system for Ehrlichia (Cowdria) ruminantium comb. nov. was first established in 1985 and many stocks were subsequently isolated and propagated in vitro. A notable exception, however, was the Kumm isolate ...
  • Latif, Abdalla A.; Steyn, Helena C.; Josemans, Antoinette I.; Marumo, Ratselane D.; Pretorius, Alri; Troskie, P. Christo; Combrink, Michael P.; Molepo, Lefoka C.; Haw, Anna; Mbizeni, Sikhumbuzo; Zweygarth, Erich; Mans, Ben J. (Barend Johannes) (Elsevier, 2020-11)
    Please read abstract in the article.
  • Bhoora, Raksha; Franssen, Linda; Oosthuizen, Marinda C.; Guthrie, Alan John; Zweygarth, Erich; Penzhorn, Barend Louis; Jongejan, Frans; Collins, Nicola E. (Elsevier, 2009-02)
    A molecular epidemiological survey of the protozoal parasites that cause equine piroplasmosis was conducted using samples collected from horses and zebra from different geographical locations in South Africa. A total of ...
  • Zweygarth, Erich; Nijhof, Ard M.; Knorr, Sarah; Ahmed, Jabbar S.; Al-Hosary, Amira T.A.; Obara, Isaiah; Bishop, Richard P.; Josemans, Antoinette I.; Clausen, Peter-Henning (Wiley, 2020-03)
    Theileriosis is a tick-borne disease caused by intracellular protozoa of the genus Theileria. The most important species in cattle are Theileria annulata and Theileria parva. Both species transform leucocyte host cells, ...
  • Bell-Sakyi, Lesley; Zweygarth, Erich; Blouin, Edmour F.; Gould, Ernest A.; Jongejan, Frans (Elsevier, 2007-09)
    Over 40 cell lines are currently available from 13 ixodid and one argasid tick species. The successful isolation and propagation of several economically important tick-borne pathogens in tick cell lines has created a useful ...