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Browsing by UP Author "Tuffin, Marla I."
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Ramond, Jean-Baptiste; Welz, Pamela J.; Tuffin, Marla I.; Burton, Stephanie G.; Cowan, Don A.
(Wiley-Blackwell, 2013)
AIMS : To assess the impact of winery wastewater (WW) on biological sand
filter (BSF) bacterial community structures, and to evaluate whether BSFs can
constitute alternative and valuable treatment- processes to remediate ...
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Loftie-Eaton, Wesley; Taylor, Mark; Horne, Kerry; Tuffin, Marla I.; Burton, Stephanie G.; Cowan, Don A.
(Wiley-Blackwell, 2013-04)
Geobacillus thermoglucosidasius is a Gram-positive, thermophilic bacterium capable of ethanologenic fermentation of both C5 and C6 sugars and may have possible use for commercial bioethanol production [Tang et al., 2009; ...
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Cowan, Don A.; Rybicki, Edward P.; Tuffin, Marla I.; Valverde, Angel; Wingfield, Michael J.
(AOSIS Open Journals, 2013-11)
Microorganisms inhabit virtually every possible niche on Earth, including those at the outer envelope of survival.
However, the focus of most conservation authorities and ecologists is the ‘legs and leaves’ side of biology ...
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Valverde, Angel; Tuffin, Marla I.; Cowan, Don A.
(Springer, 2012-07)
Actinobacteria are ubiquitous in soil, freshwater and marine ecosystems. Although various studies have focused on the microbial ecology of this phylum, data are scant on the ecology of actinobacteria endemic to hot springs. ...
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Aliyu, Habibu; De Maayer, Pieter; Rees, Jasper; Tuffin, Marla I.; Cowan, Don A.
(American Society for Microbiology, 2014-03)
Nesterenkonia sp. strain AN1 was isolated from Antarctic soil and is a polyextremophile, being tolerant of low temperatures,
high salt concentrations, and high alkalinity. Here we report the draft genome sequence of this strain.
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Van Zyl, Leonardo J.; Taylor, M.P.; Eley, K.; Tuffin, Marla I.; Cowan, Don A.
(Springer, 2014-02)
This study reports the expression, purification and kinetic characterization of a PDC from
Gluconobacter oxydans. Kinetic analyses showed the enzyme to have high affinity for pyruvate
(120μM at pH 5), high catalytic ...
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Makhalanyane, Thulani Peter; Valverde, Angel; Cary, Stephen Craig; Birkeland, Nils-Kare; Tuffin, Marla I.; Cowan, Don A.
(Nature Publishing Group, 2013-11)
Hypoliths (cryptic microbial assemblages that develop on the undersides of translucent rocks) are significant
contributors to regional C and N budgets in both hot and cold deserts. Previous studies in the Dry Valleys ...
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Ramond, Jean-Baptiste; Lako, Joseph D.W.; Stafford, W.H.L. (William); Tuffin, Marla I.; Cowan, Don A.
(Wiley, 2015-08)
Ammonia-oxidizing bacteria (AOB) are essential in the biogeochemical cycling of nitrogen as they catalyze the rate-limiting oxidation of ammonia into nitrite. Since their first isolation in the late 19th century, ...
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Valverde, Angel; Tuffin, Marla I.; Cowan, Don A.; Lacap, Donnabella C.; Pointing, Stephen B.
(Environmental Microbiology Reports, 2013-04)
Hypoliths, photosynthetic microbial assemblages found underneath translucent rocks, are widely distributed within the western region of the Namib Desert and other similar environments. Terminal restriction fragment length ...
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Zablocki, Olivier; Van Zyl, Leonardo J.; Adriaenssens, Evelien M.; Rubagotti, Enrico; Tuffin, Marla I.; Cary, Julie A.; Cowan, Don A.
(American Society for Microbiology, 2014-11)
The metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities,
cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded ...
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Stomeo, Francesca; Valverde, Angel; Pointing, Stephen B.; McKay, Christopher P.; Warren-Rhodes, Kimberley A.; Tuffin, Marla I.; Seely, Mary; Cowan, Don A.
(Springer, 2013-02-09)
The Namib Desert is considered the oldest desert in the world and hyperarid for the last 5 million years.
However, the environmental buffering provided by quartz and other translucent rocks supports extensive hypolithic ...
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Adriaenssens, Evelien M.; Van Zyl, Leonardo J.; De Maayer, Pieter; Rubagotti, Enrico; Rybicki, Edward P.; Tuffin, Marla I.; Cowan, Don A.
(Wiley-Blackwell, 2015-02)
Hypolithic microbial communities are specialized desert communities inhabiting the underside of translucent rocks where they are sheltered from harsh environmental conditions. Here, we present the first study of the viral ...
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Gokul, Jarishma K.; Valverde, Angel; Tuffin, Marla I.; Cary, Stephen Craig; Cowan, Don A.
(MDPI online quarterly, 2013-02-22)
The discovery of extensive and complex hypolithic communities in both cold
and hot deserts has raised many questions regarding their ecology, biodiversity and
relevance in terms of regional productivity. However, most ...
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Zablocki, Olivier; Adriaenssens, Evelien M.; Rubagotti, Enrico; Tuffin, Marla I.; Cowan, Don A.; Van Zyl, Lonnie; Cary, Stephen Craig
(Taylor and Francis, 2014-12)
The metaviromes from 2 different
Antarctic terrestrial soil niches have
been analyzed. Both hypoliths (microbial
assemblages beneath transluscent rocks)
and surrounding open soils showed a
high level diversity of ...
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Ramond, Jean-Baptiste; Makhalanyane, Thulani Peter; Tuffin, Marla I.; Cowan, Don A.
(Wiley, 2015-04)
Normalization is a procedure classically employed to detect rare sequences in cellular
expression profiles (i.e. cDNA libraries). Here, we present a normalization protocol involving
the direct treatment of extracted ...
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Anderson, Dominique; Ferreras, Eloy R.; Tuffin, Marla I.; Cowan, Don A.
(Oxford University Press, 2015-08)
Metagenomic library screening, by functional or sequence analysis, has become an
established method for the identification of novel genes and gene products, including
genetic elements implicated in microbial stress ...
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Ramond, Jean-Baptiste; Welz, Pamela J.; Le Roes-Hill, Marilize; Tuffin, Marla I.; Burton, Stephanie G.; Cowan, Don A.
(Wiley-Blackwell, 2014-03)
In this study, three biological sand filter (BSF) were contaminated with a synthetic iron- [1500 mg L⁻¹ Fe(II), 500 mg L⁻¹ Fe(III)] and sulphate-rich (6000 mg L⁻¹ SO₄²⁻) acid mine drainage (AMD) (pH = 2), for 24 days, to ...
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Ramond, Jean-Baptiste; Tuffin, Marla I.; Welz, Pamela J.; Burton, Stephanie G.; Cowan, Don A.
(Springer, 2013-10)
Agri effluents such as winery or olive mill wastewaters are characterized by high phenolic
concentrations. These compounds are highly toxic and generally refractory to biodegradation.
Biological sand filters (BSFs) ...
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Van Zyl, Leonardo J.; Schubert, Wolf-Dieter; Tuffin, Marla I.; Cowan, Don A.
(BioMed Central, 2014)
BACKGROUND: Bacterial pyruvate decarboxylases (PDC) are rare. Their role in ethanol production and in bacterially
mediated ethanologenic processes has, however, ensured a continued and growing interest. PDCs from ...
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Ohlhoff, Colin W.; Kirby, Bronwyn M.; Casanueva, Ana; Huddy, Robert J.; Bauer, Rolene; Mutepfa, David L.R.; Cowan, Don A.; Tuffin, Marla I.
(Elsevier, 2015-08)
A thermophilic compost metagenomic library constructed in Escherichia coli was functionally screenedfor novel esterases. Of the 110,592 fosmid clones screened, 25 clones demonstrated degradative activ-ity on glyceryl ...