Browsing by UP Author "Huismans, H. (Henk), 1942-"

Browsing by UP Author "Huismans, H. (Henk), 1942-"

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  • On the relationship between bluetongue, African horsesickness and reoviruses : hybridization studies 
    Huismans, H. (Henk), 1942-; Verwoerd, Daniel Wynand; Weiss, K.E. (Published by the Government Printer, Pretoria, 1969)
    The double-stranded ribonucleic acid from bluetongue virus (BTV), African horsesickness virus (AHSV) and reovirus has been tested for hybridization with messenger RNA derived from BTV and reovirus-infected cells. No ...
  • Silencing of African horse sickness virus VP7 protein expression in cultured cells by RNA interference 
    Stassen, Liesel; Huismans, H. (Henk), 1942-; Theron, Jacques (Elsevier, 2007-12)
    RNA interference (RNAi) is the process by which double-stranded RNA directs sequence-specific degradation of homologous mRNA. Short interfering RNAs (siRNAs) are the mediators of RNAi and represent powerful tools to silence ...
  • Studies on the in vitro and the in vivo transcription of the bluetongue virus genome 
    Huismans, H. (Henk), 1942-; Verwoerd, Daniel Wynand; De Kock, V.E.; De Lange, M.; Bigalke, R.D.; Howell, P.G.; Reinecke, R.K.; Walker, Jane B. (Published by the Government Printer, Pretoria, 1972)
    Bluetongue virus particles, converted to a high density form by the selective removal of two polypeptides from their protein capsids, possess RNA dependent RNA polymerase activity. The enzyme, which can be assayed by its ...
  • Subcellular localization of the nonstructural protein NS3 of African horsesickness virus 
    Huismans, H. (Henk), 1942-; Stoltz, M.A.; Van der Merwe, Christiaan F.; Coetzee, J.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1996)
    The subcellular localization of the minor nonstructural protein NS3 of African horsesickness virus (AHSV) has been investigated by means of immunogold electron-microscopical analysis. NS3 was observed in perturbed regions ...
  • Synthesis of empty African horse sickness virus particles 
    Maree, Sonja; Maree, Francois Frederick; Putterill, John F.; De Beer, Tjaart A.P.; Huismans, H. (Henk), 1942-; Theron, Jacques (Elsevier, 2016-02)
    As a means to develop African horse sickness (AHS) vaccines that are safe and DIVA compliant, we investigated the synthesis of empty African horse sickness virus (AHSV) particles. The emphasis of this study was on the ...
  • The use of recombinant DNA technology for the development of a bluetongue virus subunit vaccine 
    Huismans, H. (Henk), 1942-; Bigalke, R.D. (Published by The Government Printer, Pretoria, 1985)
    The double-stranded RNA gene coding for the surface antigen responsible for inducing neutralising antibodies has been isolated, converted to DNA, and cloned in the plasmid pBR322. So far, only plasmids containing inserts ...
  • Use of soluble African horse sickness viral protein 7 as an antigen delivery and presentation system 
    Rutkowska, Daria Anna; Meyer, Quinton Christian; Maree, Francois Frederick; Vosloo, Wilna; Fick, Wilma; Huismans, H. (Henk), 1942- (Elsevier B.V., 2011-03)
    We have investigated the use of soluble chimeric trimers of the major capsid protein VP7 of African horse sickness virus (AHSV) as a vaccine delivery system by targeting some of the natural hydrophilic loops on the VP7 ...