#Additional File 2 : Phylogenetic analyses #Alignments were generated using the online version of MAFFT v7. #Model testing was conducted using MrModelTest2 v2.4 #Bayesian analysis was performed using MrBayes v3.2.7 #The tree was visualized using FigTree v1.4.4 and Affinity Designer v1.10 #NEXUS BEGIN DATA; DIMENSIONS NTAX=15 NCHAR=1051; FORMAT DATATYPE=DNA GAP=- INTERLEAVE=No; MATRIX Contig4908_ITS --------------------------------------------aaggatcatt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggccactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcagagtttgacctcggatcaggtagggatacccgctgaacttaa----------------------------------------------------------------------------------------------------------------------------------------------------------------- EF494044_Psilopezia_deligata_strain_KH9913 tgaaagttaaagtcgtaacaaggtttccgtaggtgaacctgcggaaggatcatt--aatgagctaaatgggcg---------------------------------------------------------------------------------------------------------agagcccaagaacctcactca---------------------------------------------------------------caacccccccctgcgtacacctccatg-ttgcctccgcggaccgcgg--------gcaccgtccgca----ccgagga-----------------------------------cggtggcccccccgcgagagg-cccacacaaccaaactctagtt---agccggacagtccctggg-gcggcacgcctcgcaaaacacaataaagtgaaaactttcaacaacggatctcttggttcccgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggtcattccggggggcatgcctgttcgagcgtcg---tatagcaccctcaagccc----------------cggcttggtgt-tggcccccctgccacccggcgagagctgcggtggctaggctgaaatccatagggccag-ggataccgtcg--cggcaatggtgtagtaattcataacctgtcacctctgtcgcaaggcgggacccggcaacccacggcaggcgcgccagcgcgcaggccacaagt-tagcaaatttcgacctcggatcaggtagggatacccgctgaacttaagcatatcaaag------------------------------------------------------------------------------------------------------------------------------------------------------ EU339123_Rhizina_undulata_isolate_PDK-1 --------------------------------------------aaggatcatt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcagagtttgacctcggatcaggtagggatacccgctgaacttaa----------------------------------------------------------------------------------------------------------------------------------------------------------------- EU346951_Rhizina_undulata_isolate_PDS-5 --------------------------------------------aaggatcatt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcggcgaaatgcgataagtaatgcgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcagagtttgacctcggatcaggtagggatacccgctgaacttaa----------------------------------------------------------------------------------------------------------------------------------------------------------------- KC201234_Rhizina_undulata_voucher_KW_39968 ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------cgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcagagtttgacctcggatcaggtagggatacccgctgaacttaagcatatca--------------------------------------------------------------------------------------------------------------------------------------------------------- KJ410121_Phymatotrichopsis_omnivora_isolate_POD11 ---------------------------------------------------------ataa-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------acctatcagtgtacctctccacg-ttgcttccgtgtggcccaggggctttggccagcgatgccggctccgcgggtttcgccccccccccccaatttgttggggggggagt--tgcccggcccacgggaggaattctccaaactcttgattgta---aaaagaacgtatgccata----------tataaagataacaaaaagtttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctccggt-attccggagggcatgcctgttcgagcgtcagcataacaaaacctcaagcacccttgtgaaagagggttggcttggtcattggcggtggtaggg----gccattt-gtgccctactctgctgaaatgtatgggtggtg-tcttccctccctaggcctttgacgtagta------------------------------------------------------------------------------tggtgg---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- KJ410123_Phymatotrichopsis_omnivora_isolate_POD13 ---------------------------------------------------------ataa-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------acctatcagtgtacctctccacg-ttgcttccgtgtggc-caggggctttggccagcgatgccggctccgcgggtttcgcccccc-------atttgttggggggtgggttgtgcccggcccacgggaggaattctccaaactcttgattgaa---aaaagaacgtatgccata----------tataaag--gaaaaaaagtttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctccggt-attccggagggcatgcctgttcgagcgtcagcataacaaaacctcaagcacccttgtgaaagagggttggcttggtcattggcggtggtgggg-------cgat-tcgccccactctgctgaaatgtatgggtggcg-tcttccctccctaggcctttgacgtagta------------------------------------------------------------------------------tggtgg---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- MF992154_Rhizina_undulata_strain_BPL929 ------------------------------aggtgaacctgcggaaggatcatt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggccactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcagagtttgacctcggatcaggtagggatacccgctgaacttaagcatatcaata------------------------------------------------------------------------------------------------------------------------------------------------------ MH857650_Rhizina_undulata_culture_CBS30056 ----------------aacaaggtttccgtaggtgaacctgcggaaggatcatt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaagtttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg--------------------------------gctccggcccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattcggg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagccttctaacccccaatgcattgacttctcagagttgaccttcggatcaggtagggatacccgctgaacttaagcatatcaa-------------------------------------------------------------------------------------------------------------------------------------------------------- MK863520_Rhizina_undulata_voucher_JLF6721 ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------tttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcagagtttgacctcggatcaggtagggatacccgctgaacttaagcatatcat-------------------------------------------------------------------------------------------------------------------------------------------------------- MN047434_Rhizina_undulata_voucher_TENN-F-069463 ----------------------------------------gcggaaggatcatt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcagagtttgacctcggatcaggtagggatacccgctgaacttaagcatatcaataagcggaggaaaagaaaccaacagggattgcctcagtaacggcgagtgaagcggcaaaagctcaaatttgaaatctggcgccttcggcgtccgagttgtaatttggagaggcatcttcgggtggggcgcaggcctatgttccttggaacag MN656158_Helvella_calycina_voucher_O-253255 a-------aaagtcgtaacaaggtttccgtaggtgaacctgcggaaggattattaaaatcag--attcggttgaagaagaaacttgaaggaacgccgcggggtagcgggtcgccaaaccaaggcccggactaccggaccagcttctgggaccgggttggggtgcggtgggccggccctgtcgtccgaccgttaggcgcaggcccacggcttggctatgcgaaaccccacctcccccttcccgccaggcggcgttcccaagagcccacaccctctgcgtacataccactg-ttgcttccccggggt-------------------acccc----ccggggg-----------------aggtccaggagcacacgatgcggcccaacccaccatcgc-ctccgccgtytgaaggccagcg---agccaaggaag-------------aaggaacgagcgaggaaacgaaaaaaaaactttcaacaacggatctcttggttcccgcatcaatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcccatcgcgccccctggc-attccggggggcatgcctgttcgagcgtctcctggaagactgcttgcacga----------ctga--acccgcggtga-tggcggcggcggcgtgccccgagcg-ggcggccacctcgctggaatctattggcggactgcctgctgc----gtgcccgagcgtgatatgacgcattggtac-------tcgagcgcggcagggcagccagcc---cccggcccttgtgtcgggcggggagagcg-agacatcggaactcctcgaatkaggtagw------------------------------------------------------------------------------------------------------------tcccc------------------------------------------------------------------ MT374020_Psilopezia_deligata_voucher_FH_RH1660 --------------------------------ggtgacctgcgg-aggatcatt--aatgagctaaatgggcg---------------------------------------------------------------------------------------------------------agagcccaagaacctcactca---------------------------------------------------------------caacccccccctgcgtacacctccatg-ttgcctccgcggaccgcgg--------gcaccgtccgca----ccgagga-----------------------------------cggtggcccccccgcgagagg-cccacacaaccaaactctagtt---agccggacagtctctggg-gcggcacgcctcgcaaaacacaataaagtgaaaactttcaacaacggatctcttggttcccgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggtcattccggggggcatgcctgttcgagcgtcg---tatagcaccctcaagccc----------------cggcttggtgt-tggcccccctgccacccggcgagagctgcggtggctaggctgaaatccatagggccag-ggataccgtcg--cggcaatggtgtagtaattcataacctgtcacctctgtcgcaaggcgggacccggcaacccacggcaggcgcgccagcgcgcaggccacaagt-tagcaaatttcgacctcggatcaggtagggatacccgctgaacttaagcatatcaataagcggaggaaaagaaaccaacagggattgcctcagtaacggcgagtgaagcggcaagagctcaaatttgaaatctggcgccctcggtgctcgagttgtaatttggagaggcactttggggtgaggcgct--------------------- MZ091969_Rhizina_sp_isolate_QU0170 --------------------------------------------------catt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcaga--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- MZ091970_Rhizina_sp_isolate_WB1059 --------------------------------------------------catt--aataaa--acccgggcc---------------------------------------------------------------------------------------------------------agcgcccgcacgtttcataca-------------------------------------------------------------------acccatctgtgtacctctccaag-ttgcttccgtgcggcaaggggcgt---gccagccacgcc----ccggggg----------------------------------ctccggccccgcccacgggagg-acccagcgaactcatgtttgaa---cctcgcatgtctgacaggcgtctccggacgcgaaaaatgtaaataagttaaaactttcaacaacggatctcttggttctcgcatcgatgaagaacgcagcgaaatgcgataagtaatgtgaattgcagaattcagtgaatcatcgaatctttgaacgcacattgcgccctctggt-attccggagggcatgcctgttcgagcgtcagc-aacaaacccctcaagccc----------------aggcttggtca-tggcggcggtgggtcactattc-gg-tggcccaccccagctgaaaatcataggcggag-gtcccctcccc--ggccccggacgtagtaataaaccattcgcc--------caaaggccaggaagcgggaac-----gccagcc-tctaacccccaatgcattgac-tctcaga--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ; END; BEGIN MRBAYES; Outgroup MN656158_Helvella_calycina_voucher_O-253255; Lset applyto=(all) nucmodel=4by4 ploidy=haploid nst=6 rates=gamma; Unlink shape=(all) statefreq=(all) tratio=(all) revmat=(all) pinvar=(all); Prset applyto=(all) statefreqpr=dirichlet(1,1,1,1); Prset applyto=(all) ratepr=variable; mcmcp starttree=random nchains=4 savebrlens=yes ngen=1000000 samplefreq=100 printfreq=1000 nruns=4 Relburnin=yes Burninfrac=0.25; END;