Abstract:
Escherichia coli sequence type ST410 is an emerging carbapenemase-producing multidrug-resistant (MDR) high-risk One-Health clone with the potential to
significantly increase carbapenem resistance among E. coli. ST410 belongs to two clades
(ST410-A and ST410-B) and three subclades (ST410-B1, ST410-B2, and ST410-B3). After a
fimH switch between clades ST410-A and ST410-B1, ST410-B2 and ST410-B3 subclades
showed a stepwise progression toward developing MDR. (i) ST410-B2 initially acquired
fluoroquinolone resistance (via homologous recombination) in the 1980s. (ii) ST410-B2
then obtained CMY-2, CTX-M-15, and OXA-181 genes on different plasmid platforms
during the 1990s. (iii) This was followed by the chromosomal integration of blaCMY-2,
fstl YRIN insertion, and ompC/ompF mutations during the 2000s to create the ST410-
B3 subclade. (iv) An IncF plasmid “replacement” scenario happened when ST410-B2
transformed into ST410-B3: F36:31:A4:B1 plasmids were replaced by F1:A1:B49 plasmids
(both containing blaCTX-M-15) followed by blaNDM-5 incorporation during the 2010s.
User-friendly cost-effective methods for the rapid identification of ST410 isolates and
clades are needed because limited data are available about the frequencies and global
distribution of ST410 clades. Basic mechanistic, evolutionary, surveillance, and clinical
studies are urgently required to investigate the success of ST410 (including the ability
to acquire successive MDR determinants). Such information will aid with management
and prevention strategies to curb the spread of carbapenem-resistant E. coli. The medical
community can ill afford to ignore the spread of a global E. coli clone with the potential
to end the carbapenem era.
