Abstract:
Synthetic and untreated dyes discharged in wastewater effluents are a threat to an ecosystem.
This study investigated dye degradation and detoxification efficiency of crude lignin peroxidase
separately obtained from the cultures of Escherichia coli (LR0250096.1) and Pseudomonas aeruginosa
(CP031449.2). The ability of the crude lignin peroxidase to degrade Malachite Green (MG), Remazol
Brilliant Blue R (RBBR), Congo Red (CR), and Azure B (AZ) was evaluated at different operating
conditions (enzyme, dye, and hydrogen peroxide concentrations; pH; temperature; and contact
time). The ability of the degraded dyes to support the growth of bacteria was also investigated.
The observed optimum operating conditions for lignin peroxidase extracts of the Escherichia coli on
AZ were 20 mg/mL enzyme concentration, 50 mg/L dye, pH 7.0, temperature 50 ◦C, and 1.5 mM
hydrogen peroxide within 20–50 min of incubation time and on MG were 20 mg/mL, 50 mg/L, 9.0,
30 ◦C, 0.1 mM, and 20 min, respectively. The enzyme extract from Pseudomonas aeruginosa on AZ
demonstrated optimum operation conditions of 20 mg/mL, 50 mg/L, pH 9.0, 40 ◦C, 1.5 mM, and
50 min, respectively and on MG, they were 20 mg/mL, 50 mg/L, 6.0, 30 ◦C, 1.0 mM, and 20 min,
respectively). The prepared enzyme showed an appreciable degradative effect on CR and RBBR
compared with commercial lignin peroxidase. The degraded dyes were able to support the growth
of two Gram-positive (Bacillus cereus and Staphylococcus aureus), and two Gram-negative (Proteus
mirabilis and Escherichia coli) bacteria, indicating the efficiency and the potential use of the enzyme
complexes in the clean-up of industrial dyes’ waste.
