Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease

Show simple item record

dc.contributor.author Chandler, Jennifer C.
dc.contributor.author Jafree, Daniyal J.
dc.contributor.author Malik, Saif
dc.contributor.author Pomeranz, Gideon
dc.contributor.author Ball, Mary
dc.contributor.author Kolatsi-Joannou, Maria
dc.contributor.author Piapi, Alice
dc.contributor.author Mason, William J.
dc.contributor.author Benest, Andrew V.
dc.contributor.author Bates, David O.
dc.contributor.author Letunovska, Aleksandra
dc.contributor.author Al-Saadi, Reem
dc.contributor.author Rabant, Marion
dc.contributor.author Boyer, Olivia
dc.contributor.author Pritchard-Jones, Kathy
dc.contributor.author Winyard, Paul J.
dc.contributor.author Mason, Andrew S.
dc.contributor.author Woolf, Adrian S.
dc.contributor.author Waters, Aoife M.
dc.contributor.author Long, David A.
dc.date.accessioned 2024-10-31T07:28:20Z
dc.date.available 2024-10-31T07:28:20Z
dc.date.issued 2024-10
dc.description DATA AVAILABILITY STATEMENT : Primary sequencing data reported in this paper are available on NCBI Sequence Read Archive (SRA PRJNA928337). Processed data for scRNA-seq experiments are available on Zenodo at DOI: 10.5281/zenodo.7565867. The codes generated during this study are available at Github repository https://github.com/daniyal-jafree1995/collaborations. All other unique reagents used in this study are available from the corresponding author on reasonable request. en_US
dc.description SUPPLEMENTARY MATERIALS AND METHODS : FIGURE S1. Supporting data for single-cell RNA seq analyses. FIGURE S2. Characterisation of primary cell lines. FIGURE S3. Isolation of podocytes from scRNA-seq data from additional murine models of glomerular disease for cross-disease comparison. FIGURE S4. Dysregulated genes shown by individual sample for each human glomerular pathology (source Nephroseq). TABLE S1. Differentially expressed genes in glomerular cells in Wt1R394W/+ mice. TABLE S2. Podocyte differentially expressed genes that are conserved across murine glomerular scRNA-seq datasets or unique to Wt1R394W/+. TABLE S3. Podocyte differentially expressed genes classified in ‘GO:0002376 – immune system process’ across murine glomerular scRNA-seq datasets. en_US
dc.description.abstract Please read abstract in the article. en_US
dc.description.department Medical Oncology en_US
dc.description.librarian hj2024 en_US
dc.description.sdg SDG-03:Good heatlh and well-being en_US
dc.description.sponsorship LifeArc/Great Ormond Street Children's Charity; Medical Research Centre; Kidney Research UK; British Heart Foundation; Diabetes UK; Wellcome Trust. en_US
dc.description.uri https://onlinelibrary.wiley.com/journal/10969896 en_US
dc.identifier.citation Chandler J.C., Jafree D.J., Malik S. et al. 2024, 'Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease', Journal of Pathology, vol. 264, no. 2, pp. 212-227, doi : 10.1002/path.6339. en_US
dc.identifier.issn 0022-3417 (print)
dc.identifier.issn 1096-9896 (online)
dc.identifier.other 10.1002/path.6339
dc.identifier.uri http://hdl.handle.net/2263/98857
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.rights © 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. This is an open access article under the terms of the Creative Commons Attribution License. en_US
dc.subject Vascular endothelial growth factor-A en_US
dc.subject WT1 glomerulopathy en_US
dc.subject Wilms tumour 1 (WT1) en_US
dc.subject Single-cell transcriptomics en_US
dc.subject Podocyte en_US
dc.subject Neuropilin-1 en_US
dc.subject Glomerular endothelium en_US
dc.subject Childhood glomerular disease en_US
dc.subject Adrenomedullin en_US
dc.subject SDG-03: Good health and well-being en_US
dc.title Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record