Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease

Chandler, Jennifer C.; Jafree, Daniyal J.; Malik, Saif; Pomeranz, Gideon; Ball, Mary; Kolatsi-Joannou, Maria; Piapi, Alice; Mason, William J.; Benest, Andrew V.; Bates, David O.; Letunovska, Aleksandra; Al-Saadi, Reem; Rabant, Marion; Boyer, Olivia; Pritchard-Jones, Kathy; Winyard, Paul J.; Mason, Andrew S.; Woolf, Adrian S.; Waters, Aoife M.; Long, David A.

Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease

Chandler, Jennifer C.; Jafree, Daniyal J.; Malik, Saif; Pomeranz, Gideon; Ball, Mary; Kolatsi-Joannou, Maria; Piapi, Alice; Mason, William J.; Benest, Andrew V.; Bates, David O.; Letunovska, Aleksandra; Al-Saadi, Reem; Rabant, Marion; Boyer, Olivia; Pritchard-Jones, Kathy; Winyard, Paul J.; Mason, Andrew S.; Woolf, Adrian S.; Waters, Aoife M.; Long, David A.

URI: http://hdl.handle.net/2263/98857

Date: 2024-10

Abstract:

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Description:

DATA AVAILABILITY STATEMENT : Primary sequencing data reported in this paper are available on NCBI Sequence Read Archive (SRA PRJNA928337). Processed data for scRNA-seq experiments are available on Zenodo at DOI: 10.5281/zenodo.7565867. The codes generated during this study are available at Github repository https://github.com/daniyal-jafree1995/collaborations. All other unique reagents used in this study are available from the corresponding author on reasonable request.

SUPPLEMENTARY MATERIALS AND METHODS : FIGURE S1. Supporting data for single-cell RNA seq analyses. FIGURE S2. Characterisation of primary cell lines. FIGURE S3. Isolation of podocytes from scRNA-seq data from additional murine models of glomerular disease for cross-disease comparison. FIGURE S4. Dysregulated genes shown by individual sample for each human glomerular pathology (source Nephroseq). TABLE S1. Differentially expressed genes in glomerular cells in Wt1R394W/+ mice. TABLE S2. Podocyte differentially expressed genes that are conserved across murine glomerular scRNA-seq datasets or unique to Wt1R394W/+. TABLE S3. Podocyte differentially expressed genes classified in ‘GO:0002376 – immune system process’ across murine glomerular scRNA-seq datasets.

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