Whole genome sequence analysis of Brucella spp. from human, livestock, and wildlife in South Africa

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dc.contributor.author Mazwi, Koketso Desiree
dc.contributor.author Lekota, Kgaugelo Edward
dc.contributor.author Glover, Barbara Akofo
dc.contributor.author Kolo, Francis Babaman
dc.contributor.author Hassim, Ayesha
dc.contributor.author Rossouw, Jenny
dc.contributor.author Jonker, Annelize
dc.contributor.author Wojno, Justnya Maria
dc.contributor.author Profiti, Giuseppe
dc.contributor.author Martelli, Pier Luigi
dc.contributor.author Casadio, Rita
dc.contributor.author Zilli, Katiuscia
dc.contributor.author Janowicz, Anna
dc.contributor.author Marotta, Francesca
dc.contributor.author Garofolo, Giuliano
dc.contributor.author Van Heerden, Henriette
dc.date.accessioned 2024-10-30T12:20:54Z
dc.date.available 2024-10-30T12:20:54Z
dc.date.issued 2024-09
dc.description DATA AVAILABILITY : The genomes data is available on NCBI under project PRJNA-1097985. en_US
dc.description.abstract Brucellosis is an economically important zoonotic disease affecting humans, livestock, and wildlife health globally and especially in Africa. Brucella abortus and B. melitensis have been isolated from human, livestock (cattle and goat), and wildlife (sable) in South Africa (SA) but with little knowledge of the population genomic structure of this pathogen in SA. As whole genome sequencing can assist to differentiate and trace the origin of outbreaks of Brucella spp. strains, the whole genomes of retrospective isolates (n = 19) from previous studies were sequenced. Sequences were analysed using average nucleotide identity (ANI), pangenomics, and whole genome single nucleotide polymorphism (wgSNP) to trace the geographical origin of cases of brucellosis circulating in human, cattle, goats, and sable from different provinces in SA. Pangenomics analysis of B. melitensis (n = 69) and B. abortus (n = 56) was conducted with 19 strains that included B. abortus from cattle (n = 3) and B. melitensis from a human (n = 1), cattle (n = 1), goat (n = 1), Rev1 vaccine strain (n = 1), and sable (n = 12). Pangenomics analysis of B. melitensis genomes, highlighted shared genes, that include 10 hypothetical proteins and genes that encodes for acetyl-coenzyme A synthetase (acs), and acylamidase (aam) amongst the sable genomes. The wgSNP analysis confirmed the B. melitensis isolated from human was more closely related to the goat from the Western Cape Province from the same outbreak than the B. melitensis cattle sample from different cases in the Gauteng Province. The B. melitensis sable strains could be distinguished from the African lineage, constituting their own African sub-clade. The sequenced B. abortus strains clustered in the C2 lineage that is closely related to the isolates from Mozambique and Zimbabwe. This study identified genetically diverse Brucella spp. among various hosts in SA. This study expands the limited known knowledge regarding the presence of B. melitensis in livestock and humans in SA, further building a foundation for future research on the distribution of the Brucella spp. worldwide and its evolutionary background. en_US
dc.description.department Veterinary Tropical Diseases en_US
dc.description.librarian hj2024 en_US
dc.description.sdg SDG-03:Good heatlh and well-being en_US
dc.description.sponsorship Supported by the Belgian Directorate-General for Development Cooperation (DGD) within the DGD-ITM Framework Agreement 4, Gauteng Department of Agriculture and Rural Development GDARD 2016-2017, National Research Foundation, NRF grant 98651 and Erasmus plus. Open access funding provided by University of Pretoria. en_US
dc.description.uri https://www.springer.com/journal/12275 en_US
dc.identifier.citation Mazwi, K.D., Lekota, K.E., Glover, B.A. et al. Whole Genome Sequence Analysis of Brucella spp. from Human, Livestock, and Wildlife in South Africa. Journal of Microbiology 2024 Sep; 62(9): 759-773. doi: 10.1007/s12275-024-00155-8. en_US
dc.identifier.issn 1225-8873 (print)
dc.identifier.issn 1976-3794 (online)
dc.identifier.other 10.1007/s12275-024-00155-8
dc.identifier.uri http://hdl.handle.net/2263/98849
dc.language.iso en en_US
dc.publisher Springer en_US
dc.rights © 2024. The Author(s). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License. en_US
dc.subject Brucellosis en_US
dc.subject Human en_US
dc.subject Livestock en_US
dc.subject Wildlife en_US
dc.subject Brucella abortus en_US
dc.subject Brucella melitensis en_US
dc.subject SDG-03: Good health and well-being en_US
dc.subject Whole genome sequencing (WGS) en_US
dc.title Whole genome sequence analysis of Brucella spp. from human, livestock, and wildlife in South Africa en_US
dc.type Article en_US


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