dc.contributor.author |
Ibrahim, M.I.A.
|
|
dc.contributor.author |
Lensink, Antonia Vergina
|
|
dc.contributor.author |
Phaswane, Rephima M.
|
|
dc.contributor.author |
Botha, C.J. (Christoffel Jacobus)
|
|
dc.date.accessioned |
2024-09-13T04:59:45Z |
|
dc.date.available |
2024-09-13T04:59:45Z |
|
dc.date.issued |
2024-08 |
|
dc.description |
DATA AVAILABILITY :
Data will be made available on request. |
en_US |
dc.description.abstract |
Exposure to the neonicotinoid insecticide, imidacloprid (IMI), causes reproductive toxicity in mammals and reptiles. However, reports on the effects of IMI on the gonads in birds are grossly lacking. Therefore, this study investigated the effects of pubertal exposure to IMI on the histology, ultrastructure, as well as the cytoskeletal proteins, desmin, smooth muscle actin and vimentin, of the gonads of Japanese quail (Coturnix coturnix japonica). Quails were randomly divided into four groups at 5 weeks of age. The control group was given only distilled water, whereas, the other three experimental groups, IMI was administered by oral gavage at 1.55, 3.1, and 6.2 mg/kg, twice per week for 4 weeks. Exposure to IMI doses of 3.1 and 6.2 mg/kg caused dose-dependent histopathological changes in the ovary and testis. In the ovary, accumulation of lymphocytes, degenerative changes, and necrosis with granulocyte infiltrations were observed, while in the testis, distorted seminiferous tubules, germ cell sloughing, vacuolisations, apoptotic bodies, autophagosomes, and mitochondrial damage were detected. These changes were accompanied by a decreased number of primary follicles (P ≤ 0.05) in the ovary and a decrease (P ≤ 0.05) in the epithelial height, luminal, and tubular diameters of seminiferous tubules at the two higher dosages. In addition, IMI had a negative effect on the immunostaining intensity of desmin, smooth muscle actin, and vimentin in the ovarian and testicular tissue. In conclusion, exposure to IMI during puberty can lead to a range of histopathological alterations in the gonads of Japanese quails, which may ultimately result in infertility. |
en_US |
dc.description.department |
Anatomy and Physiology |
en_US |
dc.description.department |
Paraclinical Sciences |
en_US |
dc.description.librarian |
hj2024 |
en_US |
dc.description.sdg |
SDG-03:Good heatlh and well-being |
en_US |
dc.description.sponsorship |
The University of Pretoria as part of the Co-funded Post-Doctoral Fellowship program. |
en_US |
dc.description.uri |
https://www.elsevier.com/locate/tice |
en_US |
dc.identifier.citation |
Ibrahim, M.I.A., Lensink, A.V., Phaswane, R.M. & Botha, C.J. 2024, 'Structural gonadal lesions observed in Japanese quail (Coturnix coturnix japonica) following exposure during puberty to the neonicotinoid pesticide, imidacloprid', Tissue and Cell, vol. 89, art. 102450, pp. 1-14, doi : 10.1016/j.tice.2024.102450. |
en_US |
dc.identifier.issn |
0040-8166 (print) |
|
dc.identifier.issn |
1532-3072 (online) |
|
dc.identifier.uri |
http://hdl.handle.net/2263/98170 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2024 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-
nc/4.0/). |
en_US |
dc.subject |
Neonicotinoid insecticide |
en_US |
dc.subject |
Imidacloprid (IMI) |
en_US |
dc.subject |
Reproductive toxicity |
en_US |
dc.subject |
Japanese quail (Coturnix coturnix japonica) |
en_US |
dc.subject |
Ovary |
en_US |
dc.subject |
Testis |
en_US |
dc.subject |
SDG-03: Good health and well-being |
en_US |
dc.title |
Structural gonadal lesions observed in Japanese quail (Coturnix coturnix japonica) following exposure during puberty to the neonicotinoid pesticide, imidacloprid |
en_US |
dc.type |
Article |
en_US |