Molecular characterization of Leptospira species detected in the kidneys of slaughtered livestock in abattoirs in Gauteng Province, South Africa

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dc.contributor.author Dogonyaro, Banenat Bajehson
dc.contributor.author Van Heerden, Henriette
dc.contributor.author Potts, Andrew D.
dc.contributor.author Fasina, Folorunso Oludayo
dc.contributor.author Casanovas-Massana, Arnau
dc.contributor.author Kolo, Francis Babaman
dc.contributor.author Lotter, Christine
dc.contributor.author Byaruhanga, Charles
dc.contributor.author Ko, Albert I.
dc.contributor.author Wunder, Jr.Elsio A.
dc.contributor.author Adesiyun, Abiodun Adewale
dc.date.accessioned 2024-09-10T11:33:01Z
dc.date.available 2024-09-10T11:33:01Z
dc.date.issued 2023-04-30
dc.description DATA AVAILABILITY STATEMENT : Data are available in a publicly accessible repository. en_US
dc.description.abstract Leptospira was investigated in kidneys (n = 305) from slaughtered livestock in the Gauteng Province abattoirs, South Africa, using a culture medium to isolate Leptospira, followed by the LipL32 qPCR to detect Leptospira DNA. The SecY gene region was amplified, sequenced, and analyzed for LipL32 qPCR-positive samples or Leptospira isolates. The overall frequency of isolation of Leptospira spp. was 3.9% (12/305), comprising 4.8% (9/186), 4.1% (3/74), and 0% (0/45) from cattle, pigs, and sheep, respectively (p > 0.05). However, with LipL32 qPCR, the overall frequency of Leptospira DNA was 27.5%, consisting of 26.9%, 20.3%, and 42.2% for cattle, pigs, and sheep, respectively (p = 0.03). Based on 22 SecY sequences, the phylogenetic tree identified the L. interrogans cluster with serovar Icterohaemorrhagiae and the L. borgpetersenii cluster with serovar Hardjo bovis strain Lely 607. This study is the first molecular characterization of Leptospira spp. from livestock in South Africa. The reference laboratory uses an eight-serovar microscopic agglutination test panel for leptospirosis diagnosis, of which L. borgpetersenii serovar Hardjo bovis is not part. Our data show that pathogenic L. interrogans and L. borgpetersenii are circulating in the livestock population. Diagnostic use of molecular methods will eliminate or reduce the under-reporting of leptospirosis in livestock, particularly sheep, in South Africa. en_US
dc.description.department Production Animal Studies en_US
dc.description.department Veterinary Tropical Diseases en_US
dc.description.librarian am2024 en_US
dc.description.sdg SDG-02:Zero Hunger en_US
dc.description.sdg SDG-03:Good heatlh and well-being en_US
dc.description.sponsorship The Gauteng Department of Agriculture and Rural Development (GDARD), and the University of Pretoria, Postgraduate Study Abroad Bursary. en_US
dc.description.uri https://www.mdpi.com/journal/pathogens en_US
dc.identifier.citation Dogonyaro, B.B.; van Heerden, H.; Potts, A.D.; Fasina, F.O.; Casanovas-Massana, A.; Kolo, F.B.; Lötter, C.; Byaruhanga, C.; Ko, A.I.; Wunder, E.A., Jr.; et al. Molecular Characterization of Leptospira Species Detected in the Kidneys of Slaughtered Livestock in Abattoirs in Gauteng Province, South Africa. Pathogens 2023, 12, 666. https://DOI.org/10.3390/pathogens12050666. en_US
dc.identifier.issn 2076-0817 (online)
dc.identifier.other 10.3390/pathogens12050666
dc.identifier.uri http://hdl.handle.net/2263/98107
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. en_US
dc.subject Isolation en_US
dc.subject Molecular characterization en_US
dc.subject Leptospira spp. en_US
dc.subject Livestock en_US
dc.subject Abattoirs en_US
dc.subject South Africa (SA) en_US
dc.subject SDG-03: Good health and well-being en_US
dc.subject SDG-02: Zero hunger en_US
dc.title Molecular characterization of Leptospira species detected in the kidneys of slaughtered livestock in abattoirs in Gauteng Province, South Africa en_US
dc.type Article en_US


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