Chrysanthemum (Chrysanthemum morifolium Ramat.) is a globally important
ornamental plant with great economic, cultural, and symbolic value. However,
research on chrysanthemum is challenging due to its complex genetic background.
Here, we report a near-complete assembly and annotation for
C. morifolium comprising 27 pseudochromosomes (8.15 Gb; scaffold N50 of
303.69Mb). Comparative and evolutionary analyses reveal a whole-genome
triplication (WGT) event shared by Chrysanthemum species approximately 6
million years ago (Mya) and the possible lineage-specific polyploidization of
C. morifolium approximately 3 Mya. Multilevel evidence suggests that
C. morifolium is likely a segmental allopolyploid. Furthermore, a combination
of genomics and transcriptomics approaches demonstrate the C. morifolium
genome can be used to identify genes underlying key ornamental traits. Phylogenetic
analysis of CmCCD4a traces the flower colour breeding history of
cultivated chrysanthemum. Genomic resources generated from this study
could help to accelerate chrysanthemum genetic improvement.
Description:
DATA AVAILABILITY : The raw sequencing data generated in this study have been deposited
in the NCBI under accession PRJNA796762 and PRJNA895586 The
chloroplast andmitochondrial genome were also available at GenBank
under the accession number OP104251 and OP104742 respectively.
The assembled genome sequences and annotations are available at
Figshare [https://doi.org/10.6084/m9.figshare.21655364.v2]. The Arabidopsis
ABCE and chrysanthemum CYC2 genes were used as query
sequences for gene family identification, which are available at Figshare
[https://doi.org/10.6084/m9.figshare.21610305]. Source data are
provided with this paper.
