Diversity of enteroviruses in cerebrospinal fluid specimens collected from hospitalised patients in the private and public sector in South Africa

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dc.contributor.author Janse van Rensburg, Megan
dc.contributor.author Mans, Janet
dc.contributor.author Mafuyeka, Rendani T.
dc.contributor.author Strydom, Kathy-Anne
dc.contributor.author Myburgh, Marcelle
dc.contributor.author Van Zyl, Walda B.
dc.date.accessioned 2024-08-07T05:20:44Z
dc.date.available 2024-08-07T05:20:44Z
dc.date.issued 2024-03
dc.description DATA AVAILABILITY STATEMENT : The sequencing data that support the findings of this study are openly available in GenBank, reference number MZ2825208 – MZ2825290. No other data are publicly available. en_US
dc.description.abstract Enteroviruses cause a wide range of neurological illnesses such as encephalitis, meningitis, and acute flaccid paralysis. Two types of enteroviruses, echovirus E4 and E9, have recently been detected in South Africa and are known to be associated with meningitis and encephalitis. The objective of this study was to characterize enterovirus strains detected in cerebrospinal fluid specimens of hospitalized patients in the private and public sector to identify genotypes associated with meningitis and encephalitis. From January 2019 to June 2021 enterovirus positive nucleic acid samples were obtained from a private (n = 116) and a public sector (n = 101) laboratory. These enteroviruses were typed using a nested set of primers targeting the VP1 region of the enterovirus genome, followed by Sanger sequencing and BLASTn analysis. Forty-two percent (91/217) of the strains could be genotyped. Enterovirus B species was the major species detected in 95% (86/91) of the specimens, followed by species C in 3% (3/91) and species A in 2% (2/91) of the specimens. Echovirus E4 and E9 were the two major types identified in this study and were detected in 70% (64/91) and in 10% (9/91) of specimens, respectively. Echovirus E11 has previously been identified in sewage samples from South Africa, but this study is the first to report Echovirus E11 in cerebrospinal fluid specimens from South African patients. The genotypes identified during this study are known to be associated with encephalitis and meningitis. The predominant detection of echovirus E4 followed by E9 corresponds with other studies conducted in South Africa. en_US
dc.description.department Medical Virology en_US
dc.description.librarian hj2024 en_US
dc.description.sdg SDG-03:Good heatlh and well-being en_US
dc.description.sponsorship National Health Laboratory Service Research Trust and Research Committee of the University of Pretoria. en_US
dc.description.uri https://wileyonlinelibrary.com/journal/jmv en_US
dc.identifier.citation Janse van Rensburg, M., Mans, J., Mafuyeka, R.T., Strydom, K.‐A., Myburgh, M. & Van Zyl, W.B. Diversity of enteroviruses in cerebrospinal fluid specimens collected from hospitalised patients in the private and public sector in South Africa.Journal of Medical Virology 2024;96:e29514. doi:10.1002/jmv.29514. en_US
dc.identifier.issn 0146-6615 (print)
dc.identifier.issn 1096-9071 (online)
dc.identifier.other 10.1002/jmv.29514
dc.identifier.uri http://hdl.handle.net/2263/97470
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.rights © 2024 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License. en_US
dc.subject Cerebrospinal fluid en_US
dc.subject Echovirus en_US
dc.subject Enterovirus en_US
dc.subject Meningitis en_US
dc.subject South Africa (SA) en_US
dc.subject SDG-03: Good health and well-being en_US
dc.title Diversity of enteroviruses in cerebrospinal fluid specimens collected from hospitalised patients in the private and public sector in South Africa en_US
dc.type Article en_US


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