This article belongs to the Special Issue titled 'African Swine Fever Virus Transmission and Control: The Role of Wild and Domestic Suids'.
SUPPORTING INFORMATION: FILE S1: Supplementary Materials: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ani14010071/s1, Figures S1–S4: Figure S1: Agarose gel electrophoresis of p72-PCR products amplified with P1 and P2 OIE p72 screening primers (Lane m: 100 bp ladder; lane N: Negative control; lane P: Positive control; lanes 10, 36, 46, 48, 52, 58 and 59; Positive amplicons (n = 7)); Figure S2: Agarose gel electrophoresis of p54 [A], CVR-ORF [C], p72 [P] and Tk [T] gene cycle sequenced products using modified reaction conditions and demonstrating improved TK gene amplification (Lanes L = 100 bp ladder; N = Negative control; A1, P1, P6, T1, T2, T3, T4, T6 and T7 are positive amplicons from purified DNA products. The figure showed that agarose gel bands for positive cycle sequenced products are evident in lanes in A1, P1, P6, T1, T2, T3, T4, T6 and T7. Figure 3 shows the second PCR results in lanes A2, A6, A7, C1, C2, C5, C6, C7, P1, P2, P5, P6, P7, T2, T5, T6 and T7); Figure S3: Agarose gel electrophoresis of p54 [A], CVR-ORF [C], p72 [P] and Tk [T] gene products (Lanes L = 100 bp ladder; N = Negative control; A2, A6, A7, C1, C2, C5, C6, C7, P1, P2, P5, P6, P7, T2,T5, T6 and T7 are positive amplicons from purified DNA products); Figure S4: Agarose gel electrophoresis of TK gene-PCR products amplified with TK-1 + TK-Rev primers (Lane L = 100 bp ladder; N = Negative control; P1= Positive control; 2, 15 and 17 are new Tk gene positive amplicons).
DATA AVAILABILITY STATEMENT : All supporting data used in this research are freely available as Supplementary Materials or at the UPeTD (https://repository.up.ac.za/handle/2263/31741, accessed on 15 December 2023). All sequence data are available in the manuscript with their Accession numbers.
