Changes in testicular histomorphometry and ultrastructure of Leydig cells in adult male Japanese quail exposed to di (n-butyl) phthalate (DBP) during the prepubertal period

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dc.contributor.author Bello, Umar M.
dc.contributor.author Madekurozwa, Mary-Catherine
dc.contributor.author Groenewald, Hermanus B.
dc.contributor.author Arukwe, Augustine
dc.contributor.author Aire, Tom A.
dc.date.accessioned 2024-05-28T11:46:01Z
dc.date.available 2024-05-28T11:46:01Z
dc.date.issued 2023-04
dc.description DATA AVAILABILITY : The data that support the findings of this study are available from the corresponding author (UMB), upon reasonable request. en_US
dc.description.abstract Phthalate esters, such as di(n-butyl) phthalate, (DBP), are synthetic chemical pollutants commonly used as plasticizers in the manufacture of plastics. In the present study, we investigated the effects of DBP in the testes of adult male quails (Coturnix cortunix japonica) exposed by oral gavage to variable doses of DBP (0 [control], 1, 10, 50, 200, and 400 mg/ kgbw−d), for 30 days during the prepubertal period, using histo-morphometric and ultrastructural techniques. Generally, significant decreases in seminiferous tubular diameter (STD) and epithelial height (SEH) were observed predominantly at the highest DBP doses (200 and 400 mg/kg), as compared to medium (50 mg/kg), and lowest doses (1 and 10 mg/kg) as well as the control group. Ultrastructurally, apparent dose-specific degenerative changes were observed in the Leydig cells. The lowest DBP doses (1 and 10 mg/kg) did not produce significant effects on Leydig cell ultrastructure, whereas, at the highest doses (200 and 400 mg/kg), the Leydig cells were remarkably conspicuous in the interstitium and appeared foamy. There was a preponderance of electron-lucent lipid droplets which crowded out the normal organelles of the cell, as well as increases in the number of dense bodies in the cytoplasm. The smooth endoplasmic reticulum (sER) was less obvious, compacted, and wedged between the abundant lipid droplets and mitochondria. Taken together, these findings indicate that pre-pubertal exposure of precocious quail birds to DBP, produced parameter-specific histometric tubular changes, as well as dose-dependent cyto-structural derangement of the Leydig cells; which consequently may lead to overt reproductive impairments in the adult bird in the environment. en_US
dc.description.department Anatomy and Physiology en_US
dc.description.librarian am2024 en_US
dc.description.sdg SDG-03:Good heatlh and well-being en_US
dc.description.sdg SDG-09: Industry, innovation and infrastructure en_US
dc.description.sponsorship FUNDING : Open access funding provided by University of Pretoria. This study was supported by research grants to UMB, from the University of Pretoria and South African Veterinary Foundation (SAVF)/Norvatis Wildlife Foundation fund. en_US
dc.description.uri https://www.springer.com/journal/11356 en_US
dc.identifier.citation Bello, U.M., Madekurozwa, M.-C., Groenewald, H.B. et al. 2023, 'Changes in testicular histomorphometry and ultrastructure of Leydig cells in adult male Japanese quail exposed to di (n-butyl) phthalate (DBP) during the prepubertal period', Environmental Science and Pollution Research, vol. 30, pp. 55402-55413. https://DOI.org/10.1007/s11356-023-25767-2. en_US
dc.identifier.issn 0944-1344 (print)
dc.identifier.issn 1614-7499 (online)
dc.identifier.other 10.1007/s11356-023-25767-2
dc.identifier.uri http://hdl.handle.net/2263/96272
dc.language.iso en en_US
dc.publisher Springer en_US
dc.rights © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License. en_US
dc.subject Di(n-butyl) phthalate en_US
dc.subject Male Japanese quails en_US
dc.subject Leydig cell en_US
dc.subject Ultrastructure en_US
dc.subject Microstereology en_US
dc.subject Endocrine disruption en_US
dc.subject Japanese quail (Coturnix coturnix japonica) en_US
dc.subject SDG-03: Good health and well-being en_US
dc.subject SDG-09: Industry, innovation and infrastructure en_US
dc.title Changes in testicular histomorphometry and ultrastructure of Leydig cells in adult male Japanese quail exposed to di (n-butyl) phthalate (DBP) during the prepubertal period en_US
dc.type Article en_US


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