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dc.contributor.author | Basson, Charlise![]() |
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dc.contributor.author | Phiri, Avulundiah Edwin![]() |
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dc.contributor.author | Gandhi, Manjunath![]() |
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dc.contributor.author | Anguelov, Roumen![]() |
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dc.contributor.author | Serem, June Cheptoo![]() |
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dc.contributor.author | Bipath, Priyesh![]() |
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dc.contributor.author | Hlophe, Yvette Nkondo![]() |
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dc.date.accessioned | 2024-05-15T05:39:19Z | |
dc.date.available | 2024-05-15T05:39:19Z | |
dc.date.issued | 2024-06 | |
dc.description | DATA AVAILABILITY STATEMENT : The dataset(s) supporting the conclusions of this article is(are) included within the article. | en_US |
dc.description.abstract | CTCE-9908, a CXC chemokine receptor 4 (CXCR4) antagonist, prevents CXCR4 phosphorylation and inhibits the interaction with chemokine ligand 12 (CXCL12) and downstream signalling pathways associated with metastasis. This study evaluated the in vitro effects of CTCE-9908 on B16 F10 melanoma cells with the use of mathematical modelling. Crystal violet staining was used to construct a mathematical model of CTCE-9908 B16 F10 (melanoma) and RAW 264.7 (non-cancerous macrophage) cell lines on cell viability to predict the half-maximal inhibitory concentration (IC50). Morphological changes were assessed using transmission electron microscopy. Flow cytometry was used to assess changes in cell cycle distribution, apoptosis via caspase-3, cell survival via extracellular signal-regulated kinase1/2 activation, CXCR4 activation and CXCL12 expression. Mathematical modelling predicted IC50 values from 0 to 100 h. At IC50, similar cytotoxicity between the two cell lines and ultrastructural morphological changes indicative of cell death were observed. At a concentration 10 times lower than IC50, CTCE-9908 induced inhibition of cell survival (p = 0.0133) in B16 F10 cells but did not affect caspase-3 or cell cycle distribution in either cell line. This study predicts CTCE-9908 IC50 values at various time points using mathematical modelling, revealing cytotoxicity in melanoma and non-cancerous cells. CTCE-9908 significantly inhibited melanoma cell survival at a concentration 10 times lower than the IC50 in B16 F10 cells but not RAW 264.7 cells. However, CTCE-9908 did not affect CXCR4 phosphorylation, apoptosis,\ or cell cycle distribution in either cell line. | en_US |
dc.description.department | Anatomy | en_US |
dc.description.department | Mathematics and Applied Mathematics | en_US |
dc.description.department | Physiology | en_US |
dc.description.librarian | hj2024 | en_US |
dc.description.sdg | SDG-03:Good heatlh and well-being | en_US |
dc.description.sponsorship | National Research Foundation; Struwig-Germeshuysen Kankernavorsingstrust; School of Medicine Research Committee (RESCOM); Research Development Program. | en_US |
dc.description.uri | http://wileyonlinelibrary.com/journal/cep | en_US |
dc.identifier.citation | Basson, C., Phiri, A.E., Gandhi, M., et al. In vitro effects and mathematical modelling of CTCE-9908 (a chemokine receptor 4 antagonist) on melanoma cell survival. Clinical and Experimental Pharmacology and Physiology 2024; 51(6): e13865. doi: 10.1111/1440-1681.13865. | en_US |
dc.identifier.issn | 0305-1870 (print) | |
dc.identifier.issn | 1440-1681 (online) | |
dc.identifier.other | 10. 1111/1440-1681.13865 | |
dc.identifier.uri | http://hdl.handle.net/2263/95973 | |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.rights | © 2024 The Authors. Clinical and Experimental Pharmacology and Physiology published by John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License. | en_US |
dc.subject | Cell survival | en_US |
dc.subject | CTCE-9908 | en_US |
dc.subject | CXC chemokine receptor 4 (CXCR4) | en_US |
dc.subject | Mathematical modelling | en_US |
dc.subject | Melanoma | en_US |
dc.subject | Chemokine ligand 12 (CXCL12) | en_US |
dc.subject.other | Health sciences articles SDG-03 | |
dc.subject.other | SDG-03: Good health and well-being | |
dc.title | In vitro effects and mathematical modelling of CTCE-9908 (a chemokine receptor 4 antagonist) on melanoma cell survival | en_US |
dc.type | Article | en_US |