Unravelling the diversity of Anaplasma species circulating in selected African wildlife hosts by targeted 16S microbiome analysis

Show simple item record

dc.contributor.author Makgabo, Sekgota Marcus
dc.contributor.author Brayton, Kelly A.
dc.contributor.author Oosthuizen, Marinda C.
dc.contributor.author Collins, Nicola E.
dc.date.accessioned 2024-05-14T12:04:43Z
dc.date.available 2024-05-14T12:04:43Z
dc.date.issued 2023
dc.description DATA AVAILABILITY : All of the sequence data generated from this study have been registered in GenBank under the BioProject accession number: PRJNA965916. Anaplasma 16S rRNA sequences were deposited under accession numbers OQ909436 to OQ909508. Additional data will be made available on request. en_US
dc.description Corrigendum to “Unravelling the diversity of Anaplasma species circulating in selected African wildlife hosts by targeted 16S microbiome analysis” [Current Research in Microbial Sciences Volume 5 (2023) 100198] Current Research in Microbial Sciences, Volume 7, 2024, Pages 100279 S. Marcus Makgabo, Kelly A. Brayton, Marinda C. Oosthuizen, Nicola E. Collins.
dc.description.abstract Organisms in the genus Anaplasma are obligate intracellular alphaproteobacteria. Bovine anaplasmosis, predominantly caused by Anaplasma marginale, is the most prevalent tick-borne disease (TBD) of cattle worldwide. Other Anaplasma species are known to cause disease; these include A. ovis, A. platys in dogs, A. capra in goats and humans, and A. phagocytophilum in humans. The rapid advancement of next-generation sequencing technologies has led to the discovery of many novel sequences ascribed to the genus Anaplasma, with over 20 putative new species being proposed since the last formal organization of the genus. Most 16S rRNA gene surveys for Anaplasma were conducted on cattle and to a lesser extent on rodents, dogs, and ticks. Little is known about the occurrence, diversity, or impact of Anaplasma species circulating in wildlife species. Therefore, we conducted a 16S rRNA gene survey with the goal of identifying Anaplasma species in a variety of wildlife species in the Kruger National Park and neighbouring game reserves, using an unbiased 16S rRNA gene microbiome approach. An Anaplasma/Ehrlichia-group specific quantitative real-time PCR (qPCR) assay revealed the presence of Anaplasma and/or Ehrlichia species in 70.0% (21/30) of African buffalo, 86.7% (26/30) of impala, 36.7% (11/30) of greater kudu, 3.2% (1/31) of African wild dog, 40.6% (13/32) of Burchell’s zebra, 43.3% (13/30) of warthog, 22.6% (7/ 31) of spotted hyena, 40.0% (12/30) of leopard, 17.6% (6/34) of lion, 16.7% (5/30) of African elephant and 8.6% (3/35) of white rhinoceros samples. Microbiome sequencing data from the qPCR positive samples revealed four 16S rRNA sequences identical to previously published Anaplasma sequences, as well as nine novel Anaplasma 16S genotypes. Our results reveal a greater diversity of putative Anaplasma species circulating in wildlife than currently classified within the genus. Our findings highlight a potential expansion of the Anaplasma host range and the need for more genetic information from other important genes or genome sequencing of putative novel species for correct classification and further assessment of their occurrence in wildlife, livestock and companion animals. en_US
dc.description.department Centre for Veterinary Wildlife Studies en_US
dc.description.department Veterinary Tropical Diseases en_US
dc.description.librarian am2024 en_US
dc.description.sdg SDG-15:Life on land en_US
dc.description.sponsorship The National Research Foundation of South Africa; the Agricultural Sector Education and Training Authority (AgriSETA); the Belgian Directorate-General for Development Cooperation through its Framework Agreement with the Institute for Tropical Medicine, and the Department of Agriculture, Land Reform and Rural Development. en_US
dc.description.uri https://www.sciencedirect.com/journal/current-research-in-microbial-sciences en_US
dc.identifier.citation Makgabo, S.M., Brayton, K.A., Oosthuizen, M.C. & Collins, N.E. 2023, 'Unravelling the diversity of Anaplasma species circulating in selected African wildlife hosts by targeted 16S microbiome analysis', Current Research in Microbial Sciences, vol. 5, art. 100198, pp. 1-12. https://DOI.org/10.1016/j.crmicr.2023.100198. en_US
dc.identifier.issn 2666-5174
dc.identifier.other 10.1016/j.crmicr.2023.100198
dc.identifier.uri http://hdl.handle.net/2263/95965
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2023 The Authors. This is an open access article under the CC BY-NC-ND license. en_US
dc.subject Anaplasma en_US
dc.subject Wildlife hosts en_US
dc.subject Diversity en_US
dc.subject Microbiome en_US
dc.subject Kruger National Park (KNP) en_US
dc.subject Kruger National Park (South Africa) en_US
dc.subject Next-generation sequencing (NGS) en_US
dc.subject Tick-borne disease (TBD) en_US
dc.subject SDG-15: Life on land en_US
dc.title Unravelling the diversity of Anaplasma species circulating in selected African wildlife hosts by targeted 16S microbiome analysis en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record