25-Hydroxycholecalciferol inhibits cell growth and induces apoptosis in SiHa cervical cells via autocrine vitamin D metabolism

Show simple item record

dc.contributor.author Punchoo, Rivak
dc.contributor.author Dreyer, Greta
dc.contributor.author Pillay, Tahir S.
dc.date.accessioned 2023-11-30T12:43:24Z
dc.date.available 2023-11-30T12:43:24Z
dc.date.issued 2023-03
dc.description DATA AVAILABILITY STATEMENT : The study data can be made available upon request to the corresponding author. The data presented in this study are available upon request from the corresponding author. The data are not publicly available due to the institutional restrictions regarding the current data submission and findings for degree purposes. en_US
dc.description.abstract Preclinical studies show that the anticancer actions of vitamin D metabolites are mediated by apoptosis, inhibition of cell proliferation and induction of cell cycle arrest. Cervical cancer cells express an autocrine vitamin D metabolising system (VDMS) comprised of a vitamin D receptor, vitamin D catabolic enzyme (CYP24A1), and the activating enzyme of 25-hydroxycholecalciferol (25(OH)D3), CYP27B1. We assessed the anticancer effects of 25(OH)D3 at clinically relevant concentrations on a cervical squamous cell cancer cell line, SiHa. We evaluated cell health parameters (cell count, viability, and cell cycle), cell death modes (apoptosis, autophagic-dependent death, and necrosis by flow cytometry and transmission electron microscopy), and autocrine VDMS gene and protein expression by qPCR and Western blot, respectively. Our study demonstrates that physiological and supraphysiological doses of 25(OH)D3 inhibit cell growth and viability and induce biochemical and morphological apoptosis in SiHa cells. These growth effects are mediated by alteration in the VDMS gene and protein expression, with prominent negative feedback at supraphysiological treatment dose. These data identify promising therapeutic potential of 25(OH)D3 in cervical cancer, which warrants further clinical translational investigations. en_US
dc.description.department Chemical Pathology en_US
dc.description.department Obstetrics and Gynaecology en_US
dc.description.sdg SDG-03:Good heatlh and well-being en_US
dc.description.sponsorship The National Research Foundation (NRF), The Research Committee (School of Medicine) of the University of Pretoria, the Research Development Program of the University of Pretoria and the South African Medical Research Council. en_US
dc.description.uri https://www.mdpi.com/journal/biomedicines en_US
dc.identifier.citation Punchoo, R.; Dreyer, G.; Pillay, T.S. 25-Hydroxycholecalciferol Inhibits Cell Growth and Induces Apoptosis in SiHa Cervical Cells via Autocrine Vitamin D Metabolism. Biomedicines 2023, 11, 871. https://doi.org/10.3390/biomedicines11030871. en_US
dc.identifier.issn 2227-9059 (print)
dc.identifier.other 10.3390/biomedicines11030871
dc.identifier.uri http://hdl.handle.net/2263/93572
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). en_US
dc.subject Cervical cancer en_US
dc.subject SiHa en_US
dc.subject 25-hydroxycholecalciferol en_US
dc.subject Apoptosis en_US
dc.subject 24-hydroxylase (CYP24A1) en_US
dc.subject 1-Alpha hydroxylase (CYP27B1) en_US
dc.subject Vitamin D receptor (VDR) en_US
dc.subject SDG-03: Good health and well-being en_US
dc.subject Vitamin D metabolising system (VDMS) en_US
dc.subject.other Health sciences articles SDG-03
dc.subject.other SDG-03: Good health and well-being
dc.title 25-Hydroxycholecalciferol inhibits cell growth and induces apoptosis in SiHa cervical cells via autocrine vitamin D metabolism en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record