DATA AVAILABILITY STATEMENT : Sequencing data of P. trichocarpa GRX genes can be found in the GenBank database under the accession numbers listed in Table S1. Sequencing data of other 13 land plant GRX genes can be found in the Phytozome and congenie databases under the accession numbers listed in Table S2. All the other relevant data can be found within the paper and its supporting information.
SUPPORTING INFORMATION : FIGURE S1. Genomic localization of full-length Populus GRX genes. FIGURE S2. Subcellular localizations of Populus GRX proteins. FIGURE S3. SDS-PAGE of Populus GRXs and mutants of PtGRXB4. FIGURE S4. Predicted structure of PtGRXB4 in complex with its substrate by molecular docking. FIGURE S5. Modified pCAMBIA1302 vector. FIGURE S6. Modified pMAL-c5x vector.
TABLE S1. Full-length GRX genes identified from the Populus trichocarpa genome.
TABLE S2. Full-length GRXs identified from 13 land plant species.
TABLE S3. Enzymatic activities of PtGRXB4 with different tags.
TABLE S4. Enzymatic activities of PtGRXB4 and its mutants shown in Figure 7a.
TABLE S5. Primers used to construct the vectors for analysis of the subcellular localization of Populus GRXs.
TABLE S6. Primers used to construct protein expression vectors for Populus GRXs.
TABLE S7. Primers used to amplify Populus mutated GRX genes and construct mutated protein expression vectors.
DATA S1. Sequence alignment used to construct the phylogenetic tree in Figure 1. DATA S2. Sequence alignment used to construct the phylogenetic tree in Figure 2a.
