dc.contributor.author |
Arjoonsingh, Virmal
|
|
dc.contributor.author |
Suepaul, Rod
|
|
dc.contributor.author |
Adesiyun, Abiodun Adewale
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|
dc.date.accessioned |
2023-01-18T07:14:25Z |
|
dc.date.available |
2023-01-18T07:14:25Z |
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dc.date.issued |
2023-01 |
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dc.description.abstract |
Determination of the immune response of dogs by measuring the antibody levels (utilizing MAT) and levels of cytokines (TNF-α, IL-4 and IFN-γ) post-vaccination with locally produced killed whole-celled Leptospiral vaccine and post-challenge with a locally isolated Leptospira icterohaemorrhagiae Copenhageni strain. For assessment of immunity of the vaccine serum antibodies were detected before and after vaccination and challenge in three studies. The effects of the challenge were determined by a variety of parameters including reisolation of the challenge Leptospira spp. via blood, urine, and kidney samples. The challenge strain did not produce generalised infection but elevated circulating antibody levels in both the control and vaccinated dogs in any of the three studies, however leptospires were reisolated from the urine of the control dogs but not the vaccinated dogs. Cytokine levels (TNF-α, IFN-γ and IL-4) were detected post-challenge in the vaccinated dogs to determine the immune profile response. The whole-killed cell vaccine in this study did not prevent leptospireamia but prevented leptospiruria in vaccinated dogs after a challenge with a live Leptospira icterohaemorrhagiea Copenhageni. The vaccine-challenge showed increased antibody (MAT) levels due to vaccination and infection (through challenge). Cytokine production (TNF-α, IFN-γ and IL-4) by the host immune system was observed post-challenge with live leptospires. |
en_US |
dc.description.department |
Production Animal Studies |
en_US |
dc.description.librarian |
hj2023 |
en_US |
dc.description.uri |
https://www.elsevier.com/locate/vetimm |
en_US |
dc.identifier.citation |
Arjoonsingh, V., Suepaul, R. & Adesiyun, A.A. 2023, 'Immune response at a vaccine-challenge study using beagle dogs and locally isolated Leptospira spp', Veterinary Immunology and Immunopathology, vol. 255, art. 110522, pp. 1-8, doi : 10.1016/j.vetimm.2022.110522. |
en_US |
dc.identifier.issn |
0165-2427 (print) |
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dc.identifier.issn |
1873-2534 (online) |
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dc.identifier.other |
10.1016/j.vetimm.2022.110522 |
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dc.identifier.uri |
https://repository.up.ac.za/handle/2263/88871 |
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dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2022 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Immunology and Immunopathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Veterinary Immunology and Immunopathology, vol. 255, art. 110522, pp. 1-8, 2022. doi : 10.1016/j.vetimm.2022.110522. |
en_US |
dc.subject |
Leptospira/leptospirosis |
en_US |
dc.subject |
Canine vaccine-challenge |
en_US |
dc.subject |
Cytokines |
en_US |
dc.subject |
Antibody response |
en_US |
dc.subject |
Leptospiremia |
en_US |
dc.subject |
Leptospiruria |
en_US |
dc.title |
Immune response at a vaccine-challenge study using beagle dogs and locally isolated Leptospira spp |
en_US |
dc.type |
Preprint Article |
en_US |