Immune response at a vaccine-challenge study using beagle dogs and locally isolated Leptospira spp

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dc.contributor.author Arjoonsingh, Virmal
dc.contributor.author Suepaul, Rod
dc.contributor.author Adesiyun, Abiodun Adewale
dc.date.accessioned 2023-01-18T07:14:25Z
dc.date.available 2023-01-18T07:14:25Z
dc.date.issued 2023-01
dc.description.abstract Determination of the immune response of dogs by measuring the antibody levels (utilizing MAT) and levels of cytokines (TNF-α, IL-4 and IFN-γ) post-vaccination with locally produced killed whole-celled Leptospiral vaccine and post-challenge with a locally isolated Leptospira icterohaemorrhagiae Copenhageni strain. For assessment of immunity of the vaccine serum antibodies were detected before and after vaccination and challenge in three studies. The effects of the challenge were determined by a variety of parameters including reisolation of the challenge Leptospira spp. via blood, urine, and kidney samples. The challenge strain did not produce generalised infection but elevated circulating antibody levels in both the control and vaccinated dogs in any of the three studies, however leptospires were reisolated from the urine of the control dogs but not the vaccinated dogs. Cytokine levels (TNF-α, IFN-γ and IL-4) were detected post-challenge in the vaccinated dogs to determine the immune profile response. The whole-killed cell vaccine in this study did not prevent leptospireamia but prevented leptospiruria in vaccinated dogs after a challenge with a live Leptospira icterohaemorrhagiea Copenhageni. The vaccine-challenge showed increased antibody (MAT) levels due to vaccination and infection (through challenge). Cytokine production (TNF-α, IFN-γ and IL-4) by the host immune system was observed post-challenge with live leptospires. en_US
dc.description.department Production Animal Studies en_US
dc.description.librarian hj2023 en_US
dc.description.uri https://www.elsevier.com/locate/vetimm en_US
dc.identifier.citation Arjoonsingh, V., Suepaul, R. & Adesiyun, A.A. 2023, 'Immune response at a vaccine-challenge study using beagle dogs and locally isolated Leptospira spp', Veterinary Immunology and Immunopathology, vol. 255, art. 110522, pp. 1-8, doi : 10.1016/j.vetimm.2022.110522. en_US
dc.identifier.issn 0165-2427 (print)
dc.identifier.issn 1873-2534 (online)
dc.identifier.other 10.1016/j.vetimm.2022.110522
dc.identifier.uri https://repository.up.ac.za/handle/2263/88871
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2022 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Immunology and Immunopathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Veterinary Immunology and Immunopathology, vol. 255, art. 110522, pp. 1-8, 2022. doi : 10.1016/j.vetimm.2022.110522. en_US
dc.subject Leptospira/leptospirosis en_US
dc.subject Canine vaccine-challenge en_US
dc.subject Cytokines en_US
dc.subject Antibody response en_US
dc.subject Leptospiremia en_US
dc.subject Leptospiruria en_US
dc.title Immune response at a vaccine-challenge study using beagle dogs and locally isolated Leptospira spp en_US
dc.type Preprint Article en_US


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