dc.contributor.author |
Steyrer, Christof
|
|
dc.contributor.author |
Pohlin, Friederike
|
|
dc.contributor.author |
Meyer, Leith Carl Rodney
|
|
dc.contributor.author |
Buss, Peter
|
|
dc.contributor.author |
Hooijberg, Emma Henriette
|
|
dc.date.accessioned |
2022-12-06T12:10:40Z |
|
dc.date.available |
2022-12-06T12:10:40Z |
|
dc.date.issued |
2022-06 |
|
dc.description.abstract |
BACKGROUND: Hematocrit (HCT) determination is an integral part of health and disease assessments in captive and wild white rhinoceroses. Several affordable automated hematology analyzers have been developed for in-clinic and field use and have the advantage of being able to measure a large number of additional measurands. However, the accuracy of these analyzers for rhinoceros HCT measurements has not yet been investigated. OBJECTIVES: We aimed to compare the HCT results generated by the EPOC portable analyzer system and the Abaxis VetScan HM5 with the gold standard of a manual packed cell volume (PCV) measured using the microhematocrit method. METHODS: Hematocrits were measured with the EPOC and the Abaxis VetScan HM5 (bovine setting) and compared with the PCVs of 69 white rhinoceros whole blood samples. Results were compared using Bland–Altman difference plots and PassingBablok regression analysis. A total allowable analytical error of 10% was set as the performance goal. RESULTS: A significant positive bias, with a mean of 7.7% for the EPOC and 17.9% for the Abaxis, was found compared with the manual PCV method. CONCLUSIONS: The allowable error goal of 10% was not exceeded with the EPOC analyzer. Although not analytically equivalent to the gold standard, the EPOC results could therefore be used as approximations in critical situations where manual measurements cannot be performed. The Abaxis exceeded this allowable error and overestimated HCTs in rhinoceroses. Therefore, method-specific reference intervals should be used. |
en_US |
dc.description.department |
Centre for Veterinary Wildlife Studies |
en_US |
dc.description.department |
Companion Animal Clinical Studies |
en_US |
dc.description.department |
Paraclinical Sciences |
en_US |
dc.description.librarian |
dm2022 |
en_US |
dc.description.sponsorship |
International Rhino Foundation;
Veterinary Wildlife Services, Kruger
National Park; Zebra Foundation for
Veterinary Zoological Education; South
African National Parks; University of
Pretoria |
en_US |
dc.description.uri |
http://www.wileyonlinelibrary.com/journal/vcp |
en_US |
dc.identifier.citation |
Steyrer, C., Miller, M., Hewlett, J., Buss, P. & Hooijberg, E.H. Comparison of three hematocrit
measurement methods in the southern white rhinoceros
(Ceratotherium simum simum). Veterinary Clinical Pathology 2022;51:225–
230. doi: 10.1111/vcp.13076. |
en_US |
dc.identifier.issn |
1939-165X (online) |
|
dc.identifier.issn |
0275-6382 (print) |
|
dc.identifier.other |
10.1111/vcp.13076 |
|
dc.identifier.uri |
https://repository.up.ac.za/handle/2263/88658 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley |
en_US |
dc.rights |
© 2022 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License. |
en_US |
dc.subject |
Abaxis HM5 |
en_US |
dc.subject |
EPOC |
en_US |
dc.subject |
Method comparison |
en_US |
dc.subject |
Packed cell volume |
en_US |
dc.subject |
Point-of-care |
en_US |
dc.subject |
White rhinoceros (Ceratotherium simum) |
en_US |
dc.subject |
Hematocrit (HCT) |
en_US |
dc.title |
Comparison of three hematocrit measurement methods in the southern white rhinoceros (Ceratotherium simum simum) |
en_US |
dc.type |
Article |
en_US |