Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa

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dc.contributor.advisor Van Heerden, Henriette
dc.contributor.coadvisor Kolo, Francis B.
dc.contributor.postgraduate Hausiku, Magrecia L.
dc.date.accessioned 2022-10-24T07:57:58Z
dc.date.available 2022-10-24T07:57:58Z
dc.date.created 2020
dc.date.issued 2020
dc.description Dissertation (MSc (Tropical Animal Health))--University of Pretoria, 2020. en_ZA
dc.description.abstract Background: Brucellosis is a contagious zoonotic bacterial disease of worldwide distribution and remains endemic in most low- and middle-income countries (LMICs), including South Africa. The current bovine brucellosis scheme in South Africa predominantly makes use of serological tests for diagnosis which lack 100% accuracy if the gold standard test (culturing) is not performed. Abattoirs can provide information on notifiable and zoonotic disease and can play a pivotal role in disease surveillance and monitoring. This study aimed at demonstrating the usefulness of abattoir surveillance for detection of Brucella spp. using serological, molecular and bacteriological methods. Methods: Serum and tissue samples (liver, spleen and lymph nodes) were collected from slaughter cattle at a local abattoir in Hammanskraal, Gauteng Province in September 2018. A total of 122 serum samples were screened for Brucella antibodies while molecular and bacteriological methods were used to detect Brucella spp. from tissue samples of serological positive animals. Results: The Rose Bengal test (RBT) revealed a seroprevalence of 22.1% (27/122, 95% CI = 15.1-30.5) and iELISA confirmed a seroprevalence of 9.0% (11/122, 95%CI = 4.6-15.6). Genus- specific 16S-23S rRNA interspacer region (ITS) - PCR detected Brucella DNA in 9 of the seropositive tissue samples of animals. From the 9 ITS-PCR positive animals, 44.4% (4/9) Brucella bacteria were isolated and confirmed to species level using the AMOS PCR assay. AMOS-PCR characterized the four Brucella isolates as B. abortus with one mixed culture consisting of B. abortus and B. melitensis in cattle. Conclusion: The findings conclude that abattoirs are facilities that can provide invaluable information on disease surveillance and that the gold standard procedure can be performed on collected tissue samples that yield in a more accurate diagnosis. en_ZA
dc.description.availability Unrestricted en_ZA
dc.description.degree MSc (Tropical Animal Health) en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.identifier.citation * en_ZA
dc.identifier.other A/S2021 en_ZA
dc.identifier.uri https://repository.up.ac.za/handle/2263/87901
dc.language.iso en en_ZA
dc.publisher University of Pretoria
dc.rights © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
dc.subject UCTD en_ZA
dc.subject Brucellosis en_ZA
dc.subject Abattoir en_ZA
dc.subject Serology en_ZA
dc.subject PCR en_ZA
dc.title Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa en_ZA
dc.type Dissertation en_ZA


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