Description:
Supporting Information: Table S1. Physicochemical properties and antifungal activity of OsDef2 derived peptides.
Figure S1. Effect of sodium azide on the intracellular translocation of 5-FAM penetratin. C. albicans cells were pre-incubated with 5mM NaN3 for 30 min and treated with 5-FAM penetratin. The cells were counterstained with DAPI (blue) and visualized by confocal microscopy. Scale bars are 10 μm.
Figure S2. DAPI stained images of 5-FAM-peptides in C. albicans. Cells were exposed for 50 min to 2.5 μM of 5-FAM-peptides, counterstained with DAPI (blue) and visualized by confocal microscopy. Top and bottom panel show same images at lower and higher magnification, respectively. Short arrow: fragmented nuclear DNA; long arrow: tubular-shaped DNA, in contrast to single and round nucleus observed in untreated cells. Images are representatives of three independent experiments, in duplicate. Scale bars are 10 μm.
