Evaluation of the rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test for rapid colistin resistance detection in lactose non-fermenting Gram-negative bacteria
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| dc.contributor.author | Jung, Hyunsul
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| dc.contributor.author | Pitout, Johann D.D.
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| dc.contributor.author | Mitton, Barend C.
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| dc.contributor.author | Strydom, Kathy-Anne
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| dc.contributor.author | Kingsburgh, Chanel
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| dc.contributor.author | Coetzee, Jennifer
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| dc.contributor.author | Ehlers, Marthie Magdaleen
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| dc.contributor.author | Kock, Marleen M.
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| dc.date.accessioned | 2022-06-30T08:39:16Z | |
| dc.date.available | 2022-06-30T08:39:16Z | |
| dc.date.issued | 2021-06 | |
| dc.description | TABLE S1: The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test results in comparison with the reference broth microdilution (BMD). | en_US |
| dc.description.abstract | INTRODUCTION : Colistin is one of the last-resort antibiotics for treating multidrug-resistant (MDR) or extensively drug-resistant (XDR) lactose non-fermenting Gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii. GAP STATEMENT : As the rate of colistin resistance is steadily rising, there is a need for rapid and accurate antimicrobial susceptibility testing methods for colistin. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test has recently been developed for rapid detection of colistin resistance in P. aeruginosa and A. baumannii. AIM : The present study aimed to evaluate the performance of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test in comparison with the reference broth microdilution (BMD) method. METHODOLOGY : The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test was performed using a total of 135 P. aeruginosa (17 colistin-resistant and 118 colistin-susceptible) and 66 A. baumannii isolates (32 colistin-resistant and 34 colistin-susceptible), in comparison with the reference BMD method. RESULTS : The categorical agreement of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test with the reference BMD method was 97.5 % with a major error rate of 0 % (0/152) and a very major error (VME) rate of 10.2 %. The VME rate was higher (23.5 %) when calculated separately for P. aeruginosa isolates. The overall sensitivity and specificity were 89.8 and 100 %, respectively. CONCLUSION : The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test performed better for A. baumannii than for P. aeruginosa. | en_US |
| dc.description.department | Medical Microbiology | en_US |
| dc.description.librarian | hj2022 | en_US |
| dc.description.sponsorship | The National Health Laboratory Service Research trust. | en_US |
| dc.description.uri | https://www.microbiologyresearch.org/content/journal/jmm | en_US |
| dc.identifier.citation | Jung, H., Pitout, J.D.D., Mitton, B.C. et al., Evaluation of the rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test for rapid colistin resistance detection in lactose non-fermenting Gram-negative bacteria. Journal of Medical Microbiology 2021;70:001373, DOI 10.1099/jmm.0.001373. | en_US |
| dc.identifier.issn | 0022-2615 (print) | |
| dc.identifier.issn | 1473-5644 (online) | |
| dc.identifier.other | 10.1099/jmm.0.001373 | |
| dc.identifier.uri | https://repository.up.ac.za/handle/2263/86006 | |
| dc.language.iso | en | en_US |
| dc.publisher | Microbiology Society | en_US |
| dc.rights | © 2021 The Authors. This is a open access article. | en_US |
| dc.subject | Colistin | en_US |
| dc.subject | Acinetobacter baumannii | en_US |
| dc.subject | Pseudomonas aeruginosa | en_US |
| dc.subject | Rapid resistance detection test | en_US |
| dc.subject | Multidrug-resistant (MDR) | en_US |
| dc.subject | Extensively drug-resistant (XDR) | en_US |
| dc.title | Evaluation of the rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test for rapid colistin resistance detection in lactose non-fermenting Gram-negative bacteria | en_US |
| dc.type | Article | en_US |
