Abstract:
Objective To compare ketamine-butorphanol-medetomidine (KBM) to butorphanolmidazolam-
medetomidine (BMM) for chemical capture (immobilisation) of serval (Leptailurus
serval).
Study design Blinded, randomised immobilisation trial.
Animals 23 free-ranging captures (KBM: 5 females, 6 males; mean weight 10.7 kg; BMM: 10
females, 2 males; mean weight 9.6 kg).
Methods Free-ranging serval were cage trapped and then immobilised using the randomly
assigned drug combination delivered via a blow dart into the gluteal muscles. Prior to darting,
a stress score was assigned (0: Calm; to 3: markedly stressed). The drug combinations were
dosed based on estimated body weights: KBM - 8.0, 0.4 and 0.08 mg kg-1, respectively; BMM
- 0.4, 0.3 and 0.08 mg kg-1, respectively. Time to first handling, duration of anaesthesia and
recovery times were recorded. Physiological variables were recorded at five-minute intervals
and arterial blood was sampled 20 minutes after instrumentation for arterial blood gas
analysis. Atipamezole (5 mg kg-1 medetomidine) and naltrexone (2 mg kg-1 butorphanol) were
administered intramuscularly for recovery. Data, presented using mean ± standard deviation
values, were analysed using student t-test, general linear mixed model and Spearman’s rank
correlation.
Results The dose based on actual body weights were 8.7 ± 1.5, 0.4 ± 0.08 and 0.09 ± 0.02 mg
kg-1 for KBM; and 0.5 ± 0.07, 0.4 ± 0.01 and 0.09 ± 0.05 mg kg-1 for BMM. Time to first handling
was 611 ± 165 seconds for KBM and 800 ± 228 seconds for BMM (p = 0.033). Both
combinations produced a physiological stable immobilisation that lasted for at least 35 minutes. Recovery was rapid and calm overall, but ataxia was noted in KBM. Stress score was
positively and strongly correlated to blood glucose (r2 = 0.788; p = 0.001) and temperature (r2
= 0.634; p = 0.015).
Conclusion and clinical relevance Both combinations produce similar effective
immobilisation that were physiologically stable in serval. Overall, BMM is recommended
because it is fully antagonisable. It is essential to provide a calm, quiet environment before
drug administration to avoid capture-induced hyperglycaemia and hyperthermia.
