Abstract:
In term of pharmaceutical and their intermediate compounds
analysis, UPLC/MS method is a valuable equipment to achieve
better confirmation on their biodegradation by fungi. The T47DKBluc
reporter gene assay is an appropriate tool to investigate to
removal of estrogenic and antiestrogenic activities of pharmaceuticals
and their metabolites from a synthetic wastewater. A consortium
of isolated South African indigenous fungi Aspergillus
niger, Mucor circinelloides, Trichoderma longibrachiatum, Trametes
polyzona and Rhizopus microspores was found to perform a removal
of pharmaceuticals and their metabolites and to reduce their estrogenic
activity below the limit of detection in a sequencing batch
reactor. Here are presented data regarding the phenolic compounds
list and the method validation for UPLC/MS analysis used
for selected pharmaceutical compounds namely carbamazepine,
diclofenac, ibuprofen and their metabolites, as well as the T47DKBluc
bioassay using as positive control, the agonist E2 for estrogenic
activity and the antagonist ICI 182,780 for antiestrogenic
activity. For better understanding of the data presented in this paper, please see the research paper “Removal of pharmaceutical’
estrogenic activity of sequencing batch reactor effluents assessed
in the T47DK-Bluc reporter gene assay” [1].
