The mating system of the Eucalyptus canker pathogen Chrysoporthe austroafricana and closely related species

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dc.contributor.author Kanzi, Aquillah M.
dc.contributor.author Steenkamp, Emma Theodora
dc.contributor.author Van der Merwe, Nicolaas Albertus (Albie)
dc.contributor.author Wingfield, Brenda D.
dc.date.accessioned 2020-04-22T08:03:38Z
dc.date.available 2020-04-22T08:03:38Z
dc.date.issued 2019-02
dc.description.abstract Fungi in the genus Chrysoporthe are economically important canker pathogens of commercially grown Eucalyptus species and native Myrtales. Before the current study, homothallism was widely accepted as the mating system of these species, but this hypothesis could not be fully tested. Using whole genome sequences, we characterized the MAT locus of two C. austroafricana isolates and its sibling species, C. cubensis and C. deuterocubensis. A unique MAT1-2 idiomorph containing a truncated MAT1-1-1 gene, and a MAT1-1-2 gene, was identified in one isolate of C. austroafricana and a MAT1-1 idiomorph was found in the other. The presence of a single idiomorph in each isolate suggests that this fungus is heterothallic. Screening for MAT genes in 65 C. austroafricana isolates revealed a bias towards MAT1-2 idiomorphs suggesting a recent introduction in Eucalyptus species. Chrysoporthe cubensis and C. deuterocubensis are apparently homothallic since all the expected MAT genes were identified in their genome sequences. These findings were corroborated by the expression profiles of pheromone genes and their receptors, which conformed to the expected patterns observed in heterothallic and homothallic isolates. Long terminal repeat sequences (LTRs) and specifically retrotransposons were identified in the MAT locus of C. deuterocubensis and C. cubensis, indicating that the evolution of mating systems in Chrysoporthe species could be mediated by these elements. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Forestry and Agricultural Biotechnology Institute (FABI) en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian hj2020 en_ZA
dc.description.sponsorship The University of Pretoria Research Development Programme, the DST/NRF Centre of Excellence in Tree Health Biotechnology at the Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, the National Research Foundation (NRF) (Grant number 87332), the Genomics Research Institute (GRI), University of Pretoria, and the DST-NRF SARChI Chair in Fungal Genomics. en_ZA
dc.description.uri http://www.elsevier.com/locate/yfgbi en_ZA
dc.identifier.citation Kanzi, A.M., Steenkamp, E.T., Van der Merwe, N.A. et al. 2019, 'The mating system of the Eucalyptus canker pathogen Chrysoporthe austroafricana and closely related species', Fungal Genetics and Biology, vol. 123, pp. 41-52. en_ZA
dc.identifier.issn 1087-1845 (print)
dc.identifier.issn 1096-0937 (online)
dc.identifier.other 10.1016/j.fgb.2018.12.001
dc.identifier.uri http://hdl.handle.net/2263/74341
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Fungal Genetics and Biology, vol. 123, pp. 41-52, 2019. doi : 10.1016/j.fgb.2018.12.001. en_ZA
dc.subject Long terminal repeat sequence (LTR) en_ZA
dc.subject Retrotransposons en_ZA
dc.subject Homothallic en_ZA
dc.subject Heterothallic en_ZA
dc.subject Mating systems en_ZA
dc.subject Chrysoporthe en_ZA
dc.subject MAT locus en_ZA
dc.title The mating system of the Eucalyptus canker pathogen Chrysoporthe austroafricana and closely related species en_ZA
dc.type Postprint Article en_ZA


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