Novel hybrid primers for sequencing of the mitochondrial cytochrome b gene in the rabbitfishes (Teleostei: Siganidae)
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| dc.contributor.author | Kiper, Ilkser Erdem
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| dc.contributor.author | Hoareau, Thierry B.
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| dc.date.accessioned | 2020-03-30T10:38:08Z | |
| dc.date.available | 2020-03-30T10:38:08Z | |
| dc.date.issued | 2020-06 | |
| dc.description.abstract | We describe novel hybrid primers for the successful amplification and sequencing of the mitochondrial cytochrome b (cytb) gene in the family Siganidae. We aligned 403 cytb sequences from 29 species and found multiple potential conserved hybrid primers (< twofold degeneracy). The best set was selected, and successfully amplified and sequenced ~ 1100 bp of cytb in the Siganidae. | en_ZA |
| dc.description.department | Biochemistry | en_ZA |
| dc.description.department | Genetics | en_ZA |
| dc.description.department | Microbiology and Plant Pathology | en_ZA |
| dc.description.librarian | hj2020 | en_ZA |
| dc.description.sponsorship | Ilkser Erdem Kiper was supported by a PhD scholarship from the University of Pretoria’s PhD programme; TBH was supported by the University of Pretoria’s research fellowship programme and the University of Pretoria research development programme. This project was partly funded by the Western Indian Ocean Marine Science Association (WIOMSA): MARG I/11/2013 and the South African Department of Science and Technology/National Research Foundation African Coelacanth Ecosystem Programme (ACEP) II. Sampling was funded in part by the project POE 2.10 POCT FED –FEDER ‘Biodiversité de l’Océan Indien’, IRD, SPC and SAIAB. | en_ZA |
| dc.description.uri | http://link.springer.com/journal/12686 | en_ZA |
| dc.identifier.citation | Kiper, I.E., Hoareau, T.B. Novel hybrid primers for sequencing of the mitochondrial cytochrome b gene in the rabbitfishes (Teleostei: Siganidae). Conservation Genetics Resources 12, 187–190 (2020). https://doi.org/10.1007/s12686-019-01089-9. | en_ZA |
| dc.identifier.issn | 1877-7252 (print) | |
| dc.identifier.issn | 1877-7260 (online) | |
| dc.identifier.other | 10.1007/s12686-019-01089-9 | |
| dc.identifier.uri | http://hdl.handle.net/2263/73869 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Springer | en_ZA |
| dc.rights | © Springer Science+Business Media Dordrecht 2019. The original publication is available at : http://link.springer.comjournal/12686. | en_ZA |
| dc.subject | Acanthuridae | en_ZA |
| dc.subject | Cytochrome b | en_ZA |
| dc.subject | Hybrid primers | en_ZA |
| dc.subject | Mitochondrial DNA (mtDNA) | en_ZA |
| dc.subject | Siganidae | en_ZA |
| dc.subject | Zanclidae | en_ZA |
| dc.title | Novel hybrid primers for sequencing of the mitochondrial cytochrome b gene in the rabbitfishes (Teleostei: Siganidae) | en_ZA |
| dc.type | Postprint Article | en_ZA |
