Altered genome-wide DNA methylation in peripheral blood of South African women with gestational diabetes mellitus

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dc.contributor.author Dias, Stephanie Charmaine
dc.contributor.author Adam, Sumaiya
dc.contributor.author Rheeder, Paul
dc.contributor.author Louw, Johan
dc.contributor.author Pheiffer, Carmen
dc.date.accessioned 2020-02-14T07:42:30Z
dc.date.available 2020-02-14T07:42:30Z
dc.date.issued 2019-11-20
dc.description Supplementary Material : Table S1. Genome-wide DNA methylation profiling identified 1098 differentially methylated CpG loci. Table S2. Differentially methylated CpG sites annotated to 942 unique genes. Table S3. Functional enrichment analysis identified 247 KEGG pathways. Table S4. Statistically significant KEGG pathways associated with GDM. Table S5. GO terms enriched by differentially methylated genes, categorized into 1181 biological processes, 161 molecular functions and 99 cellular components. en_ZA
dc.description.abstract Increasing evidence implicate altered DNA methylation in the pathophysiology of gestational diabetes mellitus (GDM). This exploratory study probed the association between GDM and peripheral blood DNA methylation patterns in South African women. Genome-wide DNA methylation profiling was conducted in women with (n = 12) or without (n = 12) GDM using the Illumina Infinium HumanMethylationEPIC BeadChip array. Functional analysis of di erentially methylated genes was conducted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. A total of 1046 CpG sites (associated with 939 genes) were di erentially methylated between GDM and non-GDM groups. Enriched pathways included GDM-related pathways such as insulin resistance, glucose metabolism and inflammation. DNA methylation of the top five CpG loci showed distinct methylation patterns in GDM and non-GDM groups and was correlated with glucose concentrations. Of these, one CpG site mapped to the calmodulin-binding transcription activator 1 (CAMTA1) gene, which have been shown to regulate insulin production and secretion and may o er potential as an epigenetic biomarker in our population. Further validation using pyrosequencing and conducting longitudinal studies in large sample sizes and in di erent populations are required to investigate their candidacy as biomarkers of GDM. en_ZA
dc.description.department Internal Medicine en_ZA
dc.description.department Obstetrics and Gynaecology en_ZA
dc.description.librarian am2020 en_ZA
dc.description.sponsorship This research was funded by the National Research Foundation, South Africa (Unique Grant no. 99391), and the South African Medical Research Council. en_ZA
dc.description.sponsorship The National Research Foundation, South Africa (Unique Grant no. 99391), and the South African Medical Research Council. en_ZA
dc.description.uri http://www.mdpi.com/journal/ijms en_ZA
dc.identifier.citation Dias, S., Adam, S., Rheeder, P. et al. 2019, 'Altered genome-wide DNA methylation in peripheral blood of South African women with gestational diabetes mellitus', International Journal of Molecular Sciences, vol. 20, art. 5828, pp. 1-17. en_ZA
dc.identifier.issn 1422-0067 (online)
dc.identifier.other 10.3390/ijms20235828
dc.identifier.uri http://hdl.handle.net/2263/73270
dc.language.iso en en_ZA
dc.publisher MDPI Publishing en_ZA
dc.rights © 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. en_ZA
dc.subject Molecular biomarkers en_ZA
dc.subject DNA methylation en_ZA
dc.subject South Africa (SA) en_ZA
dc.subject Gestational diabetes mellitus (GDM) en_ZA
dc.subject Calmodulin-binding transcription activator 1 (CAMTA1) en_ZA
dc.title Altered genome-wide DNA methylation in peripheral blood of South African women with gestational diabetes mellitus en_ZA
dc.type Article en_ZA


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