Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

Senamela, Tshiphiri; Kock, Marleen M.; Becker, Piet J.; Potgieter, Joachim

dc.contributor.author	Senamela, Tshiphiri
dc.contributor.author	Kock, Marleen M.
dc.contributor.author	Becker, Piet J.
dc.contributor.author	Potgieter, Joachim
dc.date.accessioned	2019-04-17T09:00:10Z
dc.date.available	2019-04-17T09:00:10Z
dc.date.issued	2018-01-31
dc.description.abstract	The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.	en_ZA
dc.description.department	Haematology	en_ZA
dc.description.librarian	am2019	en_ZA
dc.description.sponsorship	The National Health Laboratory Service research trust	en_ZA
dc.description.uri	http://www.ajlmonline.org	en_ZA
dc.identifier.citation	Senamela T, Kock M, Becker P, et al. Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay. African Journal of Laboratory Medicine 2018;7(1), a658. https://DOI.org/10.4102/ajlm.v7i1.658.	en_ZA
dc.identifier.issn	2225-2002 (print)
dc.identifier.issn	2225-2010 (online)
dc.identifier.other	10.4102/ajlm.v7i1.658
dc.identifier.uri	http://hdl.handle.net/2263/68993
dc.language.iso	en	en_ZA
dc.publisher	AOSIS Open Journals	en_ZA
dc.rights	© 2018. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.	en_ZA
dc.subject	Diagnostic laboratories	en_ZA
dc.subject	Sequencing	en_ZA
dc.subject	Polymerase chain reaction (PCR)	en_ZA
dc.subject	Detection	en_ZA
dc.subject	Janus kinase 2 V617F mutation	en_ZA
dc.subject.other	Health sciences articles SDG-03
dc.subject.other	SDG-03: Good health and well-being
dc.subject.other	Health sciences articles SDG-09
dc.subject.other	SDG-09: Industry, innovation and infrastructure
dc.subject.other	Health sciences articles SDG-17
dc.subject.other	SDG-17: Partnerships for the goals
dc.title	Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay	en_ZA
dc.type	Article	en_ZA