We are excited to announce that the repository will soon undergo an upgrade, featuring a new look and feel along with several enhanced features to improve your experience. Please be on the lookout for further updates and announcements regarding the launch date. We appreciate your support and look forward to unveiling the improved platform soon.
dc.contributor.author | Osei Sekyere, John![]() |
|
dc.date.accessioned | 2019-03-14T09:08:14Z | |
dc.date.available | 2019-03-14T09:08:14Z | |
dc.date.issued | 2019-04 | |
dc.description.abstract | Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheaper, faster, simpler, sensitive, and specific have become indispensable and urgently necessary. A thorough and systematic review of the literature available at Pubmed, ScienceDirect and Web of Science was thus undertaken to identify articles describing novel and efficient colistin resistance-and mcr gene-detecting methods. From the final 23 studies included in this review, both phenotypic and molecular tests were found. The phenotypic tests consisted of novel culture media viz., SuperPolymyxin™, CHROMagar COL-APSE and LBJMR media, commercial automated MIC-determining instruments such as MICRONAUT-S, Vitek 2, BD Phoenix, Sensititre and MicroScan, and novel assays such as Colistin MAC test, Colispot, rapid polymxin NP test (RPNP), alteration of Zeta potential, modified RPNP test, MICRONAUT-MIC Strip, MIC Test Strip, UMIC System, and Sensitest™ Colistin. Molecular diagnostics consisted of the CT103XL microarray, eazyplex® SuperBug kit, and Taqman®/SYBR Green® real-time PCR assays, with 100% sensitivity and specificity plus a shorter turnaround time (<3 hr). Based on the sensitivity, specificity, cost, required skill and turnaround time, the RPNP test and/or novel culture media is recommended for under-resourced laboratories while the Multiplex PCR or Taqman®/SYBR Green® real-time PCR assay alongside the RPNP or novel culture media is suggested for well-resourced ones. | en_ZA |
dc.description.department | Medical Microbiology | en_ZA |
dc.description.librarian | am2019 | en_ZA |
dc.description.uri | http://www.MicrobiologyOpen.com | en_ZA |
dc.identifier.citation | Osei Sekyere J. Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods. MicrobiologyOpen. 2019;e682. https://DOI.org/10.1002/mbo3.682. | en_ZA |
dc.identifier.issn | 2045-8827 (online) | |
dc.identifier.other | 10.1002/mbo3.682 | |
dc.identifier.uri | http://hdl.handle.net/2263/68664 | |
dc.language.iso | en | en_ZA |
dc.publisher | Wiley | en_ZA |
dc.rights | © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License. | en_ZA |
dc.subject | Colistin | en_ZA |
dc.subject | Detection methods | en_ZA |
dc.subject | Diagnostics | en_ZA |
dc.subject | Mcr-1 | en_ZA |
dc.subject | Polymyxin B | en_ZA |
dc.title | Mcr colistin resistance gene : a systematic review of current diagnostics and detection methods | en_ZA |
dc.type | Article | en_ZA |