Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria

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dc.contributor.author Mantip, Samuel
dc.contributor.author Quan, Melvyn
dc.contributor.author Shamaki, David
dc.contributor.author Van Vuuren, Moritz
dc.date.accessioned 2016-11-11T09:52:46Z
dc.date.available 2016-11-11T09:52:46Z
dc.date.issued 2016-08-31
dc.description.abstract Peste-des-petits-ruminants virus (PPRV) is a highly contagious, fatal and economically important viral disease of small ruminants that is still endemic and militates against the production of sheep and goats in endemic areas of the world. The aim of this study was to describe the viral strains within the country. This was carried out by collecting tissue and swab samples from sheep and goats in various agro-ecological zones of Nigeria. The phylogeny of archived PPRV strains or isolates and those circulating and causing recent outbreaks was determined by sequencing of the nucleoprotein (N)-gene. Twenty tissue and swab samples from apparently healthy and sick sheep and goats were collected randomly from 18 states, namely 3 states in each of the 6 agro-ecological zones visited. A total of 360 samples were collected. A total of 35 samples of 360 (9.7%) tested positive by reverse transcriptase–polymerase chain reaction, of which 25 were from oculo-nasal swabs and 10 were from tissue samples. Neighbour-joining phylogenetic analysis using Phylogenetic Analysis Using Parsimony (PAUP) identified four different lineages, that is, lineages I, II, III and IV. Interestingly, the Nigerian strains described in this study grouped in two separate major lineages, that is, lineages II and IV. Strains from Sokoto, Oyo, Plateau and Ondo states grouped according to the historical distribution of PPRV together with the Nigerian 75/1 strain of lineage II, while other strains from Sokoto, Oyo, Plateau, Akwa-Ibom, Adamawa, Kaduna, Lagos, Bauchi, Niger and Kano states grouped together with the East African and Asian strains of lineage IV. This finding confirms that both lineage II and IV strains of PPRV are circulating in Nigeria. Previously, only strains of lineage II were found to be present in the country. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2016 en_ZA
dc.description.sponsorship Dr Mohammed S. Ahmed, Executive Director, NVRI Vom en_ZA
dc.description.uri http://www.ojvr.org en_ZA
dc.identifier.citation Mantip, S., Quan, M., Shamaki, D. & Van Vuuren, M., 2016, ‘Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria’, Onderstepoort Journal of Veterinary Research 83(1), a1163. http://dx.DOI.org/10.4102/ojvr.v83i1.1163. en_ZA
dc.identifier.issn 2071-2928 (print)
dc.identifier.issn 2071-2936 (online)
dc.identifier.other 10.4102/ojvr.v83i1.1163
dc.identifier.other 24338988000
dc.identifier.other N-9316-2014
dc.identifier.other 0000-0003-3352-8488
dc.identifier.other 7004572625
dc.identifier.other N-9080-2014
dc.identifier.uri http://hdl.handle.net/2263/57929
dc.language.iso en en_ZA
dc.publisher AOSIS Open Journals en_ZA
dc.rights © 2016. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License. en_ZA
dc.subject Sheep en_ZA
dc.subject Goats en_ZA
dc.subject Nigeria en_ZA
dc.subject Peste-des-petits-ruminants virus (PPRV) en_ZA
dc.subject Viral strains en_ZA
dc.title Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria en_ZA
dc.type Article en_ZA


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